Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries.

ABSTRACT:

Objective

The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries.

Material and methods

A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>or=1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1.

Results

Allele 1 of the study polymorphism was associated with low DMFT inde

SUBMITTER: Azevedo LF 

PROVIDER: S-EPMC5349753 | biostudies-literature | 2010 Mar-Apr

REPOSITORIES: biostudies-literature