Improving the stability of ¹¹C-labeled L-methionine with ascorbate.

ABSTRACT:

Background

Carbon-11 labeled L-methionine (¹¹C-MET) is a popular tracer used in the clinic for imaging brain tumors with positron emission tomography. However, the stability of ¹¹C-MET in its final formulation is not well documented in literature. Recently, we observed fast degradation of HPLC-purified ¹¹C-MET over time, and systematic investigation was conducted to identify the cause.

Results

In this study, we verified the degraded product as ¹¹C-labeled methionine sulfoxide (¹¹C-METSO). To minimize oxidation, ascorbate (100 ppm) was added to the HPLC eluant, and the resulting HPLC-purified ¹¹C-MET was stable in the final formulation solution without noticeable degradation for up to 1 h after the end of synth

SUBMITTER: Woods M 

PROVIDER: S-EPMC5824703 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature