InsP₃R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca²⁺ elevation and TBK1 activity.

ABSTRACT:

Background

Candida albicans (C. albicans) invasion triggers antifungal innate immunity, and the elevation of cytoplasmic Ca²⁺ levels via the inositol 1,4,5-trisphosphate receptor (InsP₃R) plays a critical role in this process. However, the molecular pathways linking the InsP₃R-mediated increase in Ca²⁺ and immune responses remain elusive.

Results

In the present study, we find that during C. albicans phagocytosis in macrophages, exocyst complex component 2 (SEC5) promotes InsP₃R channel activity by binding to its C-terminal α-helix (H1), increasing cytosolic Ca²⁺ concentrations ([Ca²⁺]_c). Immunofluorescence reveals enriched InsP₃R-SEC5 complex formation on phagosomes, while disr

SUBMITTER: Yang L 

PROVIDER: S-EPMC5921305 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature