Metabolism of hyperpolarized ¹³ C-acetoacetate to β-hydroxybutyrate detects real-time mitochondrial redox state and dysfunction in heart tissue.

ABSTRACT: Mitochondrial dysfunction is considered to be an important component of many metabolic diseases yet there is no reliable imaging biomarker for monitoring mitochondrial damage in vivo. A large prior literature on inter-conversion of β-hydroxybutyrate and acetoacetate indicates that the process is mitochondrial and that the ratio reflects a specifically mitochondrial redox state. Therefore, the conversion of [1,3-¹³ C]acetoacetate to [1,3-¹³ C]β-hydroxybutyrate is expected to be sensitive to the abnormal redox state present in dysfunctional mitochondria. In this study, we examined the conversion of hyperpolarized (HP) ¹³ C-acetoacetate (AcAc) to ¹³ C-β-hydroxybutyrate (β-HB) as a potential imaging biomarker for mitochondrial redox and dysfunction i

SUBMITTER: Chen W 

PROVIDER: S-EPMC6525062 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature