Investigating LGALS3BP/90?K glycoprotein in the cerebrospinal fluid of patients with neurological diseases.

ABSTRACT: Galectin-3 binding protein (LGALS3BP or 90?K) is a secreted glycoprotein found in human body fluids. Deregulated levels were observed in cancer and infection and its study in neurological diseases is more recent. Here, we have investigated 90?K from human cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS, n?=?35) and other neurological diseases (n?=?23). CSF was fractionated by ultrafiltration/size-exclusion chromatography (SEC) and eluted fractions were analysed by complementary techniques including immunoblotting, electron microscopy and nano-liquid chromatography-tandem mass spectrometry. A fraction of 90?K appeared as nanoparticles of irregular shape with heterogeneous dimensions of 15-60?nm that co-eluted with extracellular vesicles in SEC. Median levels of 90?K quantified by ELISA were not different between ALS patients (215.8?ng/ml) and controls (213.3?ng/ml) in contrast with the benchmark biomarker for ALS phosphoneurofilament heavy chain (1750 and 345?pg/ml, respectively). A multiregression model supported age is the only independent predictor of 90?K level in both groups (p?

SUBMITTER: Costa J 

PROVIDER: S-EPMC7101329 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature