Assessment of simplified methods for quantification of [¹⁸F]-DPA-714 using 3D whole-brain TSPO immunohistochemistry in a non-human primate.

ABSTRACT: The 18 kDa translocator protein (TSPO) is the main molecular target to image neuroinflammation by positron emission tomography (PET). However, TSPO-PET quantification is complex and none of the kinetic modelling approaches has been validated using a voxel-by-voxel comparison of TSPO-PET data with the actual TSPO levels of expression. Here, we present a single case study of binary classification of in vivo PET data to evaluate the statistical performance of different TSPO-PET quantification methods. To that end, we induced a localized and adjustable increase of TSPO levels in a non-human primate brain through a viral-vector strategy. We then performed a voxel-wise comparison of the different TSPO-PET quantification approaches providing parametric [¹⁸F]-DPA-714 PET images, with co

SUBMITTER: Van Camp N 

PROVIDER: S-EPMC7181080 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature