Dataset Information

Direct Comparison of [¹⁸F]F-DPA with [¹⁸F]DPA-714 and [¹¹C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

ABSTRACT:

Purpose

In this study we compared the recently developed TSPO tracer [¹⁸F]F-DPA, with [¹⁸F]DPA-714 and [¹¹C]PBR28 by performing in vivo PET imaging on the same Alzheimer's disease mouse model APP/PS1-21 (TG) and wild-type (WT) mice with all three radiotracers.

Procedures

To compare the radiotracer uptake, percentage of injected dose/mL (%ID/mL), standardized uptake value ratios to cerebellum (SUVR_CB), and voxel-wise analyses were performed.

Results

The peak uptake of [¹⁸F]F-DPA was higher than 4.3% ID/mL, while [¹⁸F]DPA-714 reached just over 3% ID/mL, and [¹¹C]PBR28 was over 4% ID/mL in only one brain region in the WT mice. The peak/60-min uptake ratios of [¹⁸F]F-DPA were significantly higher (p < 0.001) than those of [¹⁸F]DPA-714 and [¹¹C]PBR28. The differences in [¹⁸F]F-DPA SUVR_CB between WT and TG mice were highly significant (p < 0.001) in the three studied time periods after injection. [¹⁸F]DPA-714 uptake was significantly higher in TG mice starting in the 20-40-min timeframe and increased thereafter, whereas [¹¹C]PBR28 uptake became significant at 10-20 min (p < 0.05). The voxel-wise analysis confirmed the differences between the radiotracers.

Conclusions

[¹⁸F]F-DPA displays higher brain uptake, higher TG-to-WT SUVR_CB ratios, and faster clearance than [¹⁸F]DPA-714 and [¹¹C]PBR28, and could prove useful for detecting low levels of inflammation and allow for shorter dynamic PET scans.

SUBMITTER: Lopez-Picon FR

PROVIDER: S-EPMC8760190 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Publications

Direct Comparison of [<sup>18</sup>F]F-DPA with [<sup>18</sup>F]DPA-714 and [<sup>11</sup>C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

López-Picón Francisco R FR Keller Thomas T Bocancea Diana D Helin Jatta S JS Krzyczmonik Anna A Helin Semi S Damont Annelaure A Dollé Frédéric F Rinne Juha O JO Haaparanta-Solin Merja M Solin Olof O

Molecular imaging and biology 20210920 1

<h4>Purpose</h4>In this study we compared the recently developed TSPO tracer [<sup>18</sup>F]F-DPA, with [<sup>18</sup>F]DPA-714 and [<sup>11</sup>C]PBR28 by performing in vivo PET imaging on the same Alzheimer's disease mouse model APP/PS1-21 (TG) and wild-type (WT) mice with all three radiotracers.<h4>Procedures</h4>To compare the radiotracer uptake, percentage of injected dose/mL (%ID/mL), standardized uptake value ratios to cerebellum (SUVR<sub>CB</sub>), and voxel-wise analyses were perform ...[more]

PMID: 34542805

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Project description:Hypothyroidism is closely related to mental disorders, mainly depression, through an as-yet-unknown mechanism. The cerebral inflammatory immune process has been implied to play a pivotal role in the onset of affective symptoms in several conditions. In order to gain insight into the mechanism underlying the depressive behaviors in hypothyroid rats, brain microglial activation was evaluated using micro positron emission tomography imaging with a translocator protein (TSPO) radioligand. Hypothyroidism was induced in adult male Wistar rats by administration of 0.05% propylthiouracil in drinking water for five weeks. Open field, forced swimming and tail suspension tests were employed to evaluate the depressive behavior in hypothyroid rats, and the relationship between the behavioral changes and brain microglial activation was evaluated using [18F] DPA-714 micro positron emission tomography imaging. The open field test revealed significantly reduced first-minute activity and rearing behavior in the hypothyroid group, as well as significantly increased immobility in the forced swimming test and the tail suspension test. Hypothyroidism induced significantly increased microglial activation in the hippocampus. The radioligand uptake in the hippocampus correlated negatively with first-minute activity in the open field test (p < 0.05), and the radioligand uptake in the hippocampus correlated positively with changes in the immobility time in the forced swimming test and the tail suspension test (p < 0.05). Immunohistochemistry also confirmed the activation of microglia and inflammatory bodies in hypothyroid rats. The results indicate that hypothyroidism can induce depressive behavior in adult Wistar rats, microglial activation in the hippocampus plays an important role in the depressive behavior in hypothyroid rats and the inflammatory immune mechanism may underlie the behavioral abnormalities in thyroid dysfunction. Furthermore, the findings in the present study suggest there might be a common mechanism underlying depressive behavior in adult-onset hypothyroidism and depression.

Dataset Information

Direct Comparison of [¹⁸F]F-DPA with [¹⁸F]DPA-714 and [¹¹C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

Purpose

Procedures

Results

Conclusions

Publications

Direct Comparison of [<sup>18</sup>F]F-DPA with [<sup>18</sup>F]DPA-714 and [<sup>11</sup>C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Direct Comparison of [18F]F-DPA with [18F]DPA-714 and [11C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

Purpose

Procedures

Results

Conclusions

Publications

Direct Comparison of [<sup>18</sup>F]F-DPA with [<sup>18</sup>F]DPA-714 and [<sup>11</sup>C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Direct Comparison of [¹⁸F]F-DPA with [¹⁸F]DPA-714 and [¹¹C]PBR28 for Neuroinflammation Imaging in the same Alzheimer's Disease Model Mice and Healthy Controls.