Circulating CX3CR1⁺CD163⁺ M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

ABSTRACT:

Background

Vulnerable plaques have been generally recognized to play a role in the pathogenesis of acute myocardial infarction (AMI), however, the role of circulating CX3CR1⁺CD163⁺ M2 monocytes has not been studied properly. We aim to evaluate the features of CX3CR1⁺CD163⁺ M2 monocytes and its relationship with cardiac specific markers in AMI patients.

Methods

The circulating M2 monocytes were identified in AMI patients (n=35) and healthy controls (HCs, n=10) by flow cytometry using two staining methods: CD68⁺CD163⁺ (cytoplasmic staining) and CX3CR1⁺CD163⁺ (surface staining). CX3CR1⁺ monocytes were purified by magnetic cell sorting. The expression level of peroxisome proliferat

SUBMITTER: Shao X 

PROVIDER: S-EPMC7290533 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature