Dataset Information

Circulating CX3CR1⁺CD163⁺ M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

ABSTRACT:

Background

Vulnerable plaques have been generally recognized to play a role in the pathogenesis of acute myocardial infarction (AMI), however, the role of circulating CX3CR1⁺CD163⁺ M2 monocytes has not been studied properly. We aim to evaluate the features of CX3CR1⁺CD163⁺ M2 monocytes and its relationship with cardiac specific markers in AMI patients.

Methods

The circulating M2 monocytes were identified in AMI patients (n=35) and healthy controls (HCs, n=10) by flow cytometry using two staining methods: CD68⁺CD163⁺ (cytoplasmic staining) and CX3CR1⁺CD163⁺ (surface staining). CX3CR1⁺ monocytes were purified by magnetic cell sorting. The expression level of peroxisome proliferator-activated receptor γ (PPARγ) and arginase-1 (Arg-1) were measured by real-time quantitative PCR and Western Blot in CX3CR1⁺ monocytes.

Results

Circulating M2 monocytes extremely expanded in AMI patients compared with HCs (P<0.01). Positive linear correlation was confirmed between CD68⁺CD163⁺ and CX3CR1⁺CD163⁺ cell populations in AMI patients (r=0.39, P=0.02). The percentage of circulating CX3CR1⁺CD163⁺ M2 monocytes positively correlated with cardiac specific markers (cTNT, CK-MB) and acute phase markers (glucose, hs-CRP) (cTNT, r=0.63, P<0.01, CK-MB, r=0.54, P<0.01, glucose, r=0.62, P<0.01, hs-CRP, r=0.58, P<0.01). CX3CR1⁺ monocytes in AMI patients expressed higher levels of PPARγ and Arg-1 than those in HCs (P<0.01).

Conclusions

Circulating M2 monocytes increased in AMI patients and positively correlated with the elevation of both cardiac specific and acute phase markers. CX3CR1⁺CD163⁺ M2 monocytes might have application value for the early diagnosis of AMI.

SUBMITTER: Shao X

PROVIDER: S-EPMC7290533 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

Circulating CX3CR1<sup>+</sup>CD163<sup>+</sup> M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

Shao Xia X Wu Boting B Chen Pu P Hua Fanli F Cheng Luya L Li Feng F Zhan Yanxia Y Liu Chanjuan C Ji Lili L Min Zhihui Z Sun Lihua L Cheng Yunfeng Y Chen Hao H

Annals of translational medicine 20200501 9

<h4>Background</h4>Vulnerable plaques have been generally recognized to play a role in the pathogenesis of acute myocardial infarction (AMI), however, the role of circulating CX3CR1<sup>+</sup>CD163<sup>+</sup> M2 monocytes has not been studied properly. We aim to evaluate the features of CX3CR1<sup>+</sup>CD163<sup>+</sup> M2 monocytes and its relationship with cardiac specific markers in AMI patients.<h4>Methods</h4>The circulating M2 monocytes were identified in AMI patients (n=35) and health ...[more]

PMID: 32566605

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Project description:BackgroundSystemic sclerosis (SSc) is an autoimmune disease characterized by overproduction of extracellular matrix (ECM) and multiorgan fibrosis. Animal studies pointed to bone marrow-derived cells as a potential source of pathological ECM-producing cells in immunofibrotic disorders. So far, involvement of monocytes and macrophages in the fibrogenesis of SSc remains poorly understood.Methods and resultsImmunohistochemistry analysis showed accumulation of CD14+ monocytes in the collagen-rich areas, as well as increased amount of alpha smooth muscle actin (αSMA)-positive fibroblasts, CD68+ and mannose-R+ macrophages in the heart and lungs of SSc patients. The full genome transcriptomics analyses of CD14+ blood monocytes revealed dysregulation in cytoskeleton rearrangement, ECM remodeling, including elevated FN1 (gene encoding fibronectin) expression and TGF-β signalling pathway in SSc patients. In addition, single cell RNA sequencing analysis of tissue-resident CD14+ pulmonary macrophages demonstrated activated profibrotic signature with the elevated FN1 expression in SSc patients with interstitial lung disease. Peripheral blood CD14+ monocytes obtained from either healthy subjects or SSc patients exposed to profibrotic treatment with profibrotic cytokines TGF-β, IL-4, IL-10, and IL-13 increased production of type I collagen, fibronectin, and αSMA. In addition, CD14+ monocytes co-cultured with dermal fibroblasts obtained from SSc patients or healthy individuals acquired a spindle shape and further enhanced production of profibrotic markers. Pharmacological blockade of the TGF-β signalling pathway with SD208 (TGF-β receptor type I inhibitor), SIS3 (Smad3 inhibitor) or (5Z)-7-oxozeaenol (TGF-β-activated kinase 1 inhibitor) ameliorated fibronectin levels and type I collagen secretion.ConclusionsOur findings identified activated profibrotic signature with elevated production of profibrotic fibronectin in CD14+ monocytes and CD14+ pulmonary macrophages in SSc and highlighted the capability of CD14+ monocytes to acquire a profibrotic phenotype. Taking together, tissue-infiltrating CD14+ monocytes/macrophages can be considered as ECM producers in SSc pathogenesis.

Dataset Information

Circulating CX3CR1⁺CD163⁺ M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

Background

Methods

Results

Conclusions

Publications

Circulating CX3CR1<sup>+</sup>CD163<sup>+</sup> M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Circulating CX3CR1+CD163+ M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

Background

Methods

Results

Conclusions

Publications

Circulating CX3CR1<sup>+</sup>CD163<sup>+</sup> M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Circulating CX3CR1⁺CD163⁺ M2 monocytes markedly elevated and correlated with cardiac markers in patients with acute myocardial infarction.