New assessment of Anopheles vector species identification using MALDI-TOF MS.

ABSTRACT:

Background

Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used.

Methods

To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panel

SUBMITTER: Nabet C 

PROVIDER: S-EPMC7796594 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature