Structure of In Vitro-Synthesized Cellulose Fibrils Viewed by Cryo-Electron Tomography and ¹³C Natural-Abundance Dynamic Nuclear Polarization Solid-State NMR.

ABSTRACT: Cellulose, the most abundant biopolymer, is a central source for renewable energy and functionalized materials. In vitro synthesis of cellulose microfibrils (CMFs) has become possible using purified cellulose synthase (CESA) isoforms from Physcomitrium patens and hybrid aspen. The exact nature of these in vitro fibrils remains unknown. Here, we characterize in vitro-synthesized fibers made by CESAs present in membrane fractions of P. patens over-expressing CESA5 by cryo-electron tomography and dynamic nuclear polarization (DNP) solid-state NMR. DNP enabled measuring two-dimensional ¹³C-¹³C correlation spectra without isotope-labeling of the fibers. Results show structural similarity between in vitro fibrils and native CMF in p

SUBMITTER: Deligey F 

PROVIDER: S-EPMC9198983 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature