Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork.

ABSTRACT:

Aim

To investigate potential gene changes in trabecular meshwork (TM) induced by dexamethasone (DEX) in steroid-induced glaucoma (SIG).

Methods

The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the TM. The relationships of the differentially expressed genes (DEGs) were enriched using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In addition, the hub genes were screened by the Search Tool for the Retrieval of Interacting Genes Database (STRING) and Cytoscape tools. Finally, human TM cells (HTMCs) were treated with DEX to preliminarily explore the function of hub genes.

Results

Totally 47 DEGs, including 21 downregulated and 26 upregulated genes were identif

SUBMITTER: Wei M 

PROVIDER: S-EPMC9358186 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature