Nr1d1 Mediated Cell Senescence in Mouse Heart-Derived Sca-1⁺CD31^- Cells.

ABSTRACT:

Aim

Sca-1⁺CD31^- cells are resident cardiac progenitor cells, found in many mammalian tissues including the heart, and able to differentiate into cardiomyocytes in vitro and in vivo. Our previous work indicated that heart-derived Sca-1⁺CD31^- cells increased the Nr1d1 mRNA level of Nr1d1 with aging. However, how Nr1d1 affects the senescence of Sca-1⁺CD31^- cells.

Methods

Overexpression and knockdown of Nr1d1 in Sca-1⁺CD31^- cells and mouse cardiac myocyte (MCM) cell lines were performed by lentiviral transduction. The effects of Nr1d1 abundance on cell differentiation, proliferation, apoptosis, cell cycle, and transcriptomics were evaluated. Moreover, binding of

SUBMITTER: Pu S 

PROVIDER: S-EPMC9603916 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature