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OCT6 inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation.

ABSTRACT: As a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 ( OCT6) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of OCT6 in piPSCs. We found that piPSCs overexpressing OCT6 maintained colony morphology and pluripotency under differentiation conditions, with a similar gene expression pattern to that of non-differentiated piPSCs. Functional analysis revealed that OCT6 attenuated the adverse effects of extracellular signal-regulated kinase (ERK) signaling pathway inhibition on piPSC pluripotency by activating phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) signaling activity. Our research sheds new light on the mechanism by which OCT6 promotes PSC maintenance.

SUBMITTER: Yang XC 

PROVIDER: S-EPMC9700490 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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<i>OCT6</i> inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation.

Yang Xin-Chun XC   Wu Xiao-Long XL   Li Wen-Hao WH   Wu Xiao-Jie XJ   Shen Qiao-Yan QY   Li Yun-Xiang YX   Peng Sha S   Hua Jin-Lian JL  

Zoological research 20221101 6

As a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 ( <i>OCT6</i>) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of <i>OCT6</i> in piPSCs. We found that piPSCs overexpressing <i>OCT6</i> maintained colony morphology and pluripotency under differentiation conditions, with a similar gene  ...[more]

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