Malondialdehyde formation in HepG2 cells after compound exposure

ABSTRACT: We quantified malondialdehyde formation in maturated HepG2 cells after 24 h of compound exposure. This was done using three exposure scenarios: (1) cells receiving acute exposure; (2) cells receiving repeated exposure; (3) cells receiving repeated exposure and recovery. Lipid peroxidation was assessed by measuring malondialdehyde (MDA) levels using the thiobarbituric acid reactive substances (TBARS) assay [Wong et al. Clin Chem. 1987;33(2 Pt1):214-20]. Fluorescence was measured in the supernatant at λexc= 530 nm and λem= 575 nm using a 96-wells fluorescence plate reader. The MDA levels were quantified using a standard curve. This study was performed as part of the RISK-HUNT3R project and the PARC project.

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Jeroen Pennings 

PROVIDER: S-BSST2296 | biostudies-other |

REPOSITORIES: biostudies-other