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Ultra-sensitive profiling of CRISPR-Cas off-target effects with Tracking-seq2

ABSTRACT: Accurate detection of off-target activity in patient-derived cells is critical for ensuring the safety of gene therapeutics. Here, we present Tracking-seq2—the most sensitive, cell-based, and clinically applicable off-target detection technology to date, capable of profiling diverse genome editors. Crucially, we reveal that genomic variations drive distinct off-target heterogeneity across different cells, highlighting the necessity for personalized safety assessment in clinical genome editing therapies

ORGANISM(S): Homo sapiens

PROVIDER: GSE307225 | GEO | 2026/06/16

REPOSITORIES: GEO

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Ultra-sensitive profiling of CRISPR-Cas off-target effects with Tracking-seq2

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