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The secreted protein MoSun4 of Magnaporthe oryzae regulates pathogenicity by repressing the expression of virulence-related genes

ABSTRACT: The SUN family protein MoSun4 in Magnaporthe oryzae has been previously implicated in mitophagy and can be used as a target for reducing rice blast, but its role as a secreted protein remain poorly understood. In this study, signal peptide prediction and yeast secretion assays confirmed the activity of MoSun4 signal peptide, and co-localization revealed its extra-invasive hyphal membrane (EIHM) localization, establishing MoSun4 as a secreted protein. In addition, deletion of MoSUN4 severely attenuated the hyphal growth, conidiation and virulence of M. oryzae. We further showed that MoSun4 represses the expression of virulence-related genes, including multiple genes involved in cell wall degradation (eglC, eglD), secondary metabolism (gliK), and melanin biosynthesis (SDH1, BUF1, Cmr1, ALB1). Collectively, our study reveals that MoSun4 is a secreted protein and regulates pathogenicity by repressing the expression of virulence-related genes, providing new insights into the function of SUN family proteins.

ORGANISM(S): Pyricularia oryzae

PROVIDER: GSE330356 | GEO | 2026/05/20

REPOSITORIES: GEO

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