Proteomics

Dataset Information

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Analysis of Plasmodium sporozoites collected from mosquito saliva

ABSTRACT: Plasmodium sporozoites are injected, in addition to saliva, into animal hosts when a female Anopheles mosquito takes a blood meal. The molecular components of saliva that interact with Plasmodium during this process are poorly characterized. Here we collected Plasmodium sporozoites directly from salivating Anopheles mosquitoes and looked for the presence of vector proteins that could be interacting with the parasites during transmission for further characterization.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Anopheles Stephensi (asian Malaria Mosquito) Plasmodium Falciparum (isolate 3d7) Plasmodium Berghei Anka Anopheles Gambiae (african Malaria Mosquito)

TISSUE(S): Saliva

DISEASE(S): Malaria

SUBMITTER: Tyler Schleicher  

LAB HEAD: Erol Fikrig

PROVIDER: PXD010121 | Pride | 2018-10-18

REPOSITORIES: Pride

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Publications

A mosquito salivary gland protein partially inhibits Plasmodium sporozoite cell traversal and transmission.

Schleicher Tyler R TR   Yang Jing J   Freudzon Marianna M   Rembisz Alison A   Craft Samuel S   Hamilton Madeleine M   Graham Morven M   Mlambo Godfree G   Tripathi Abhai K AK   Li Yue Y   Cresswell Peter P   Sinnis Photini P   Dimopoulos George G   Fikrig Erol E  

Nature communications 20180725 1

The key step during the initiation of malaria is for motile Plasmodium parasites to exit the host dermis and infect the liver. During transmission, the parasites in the form of sporozoites, are injected together with mosquito saliva into the skin. However, the contribution of vector saliva to sporozoite activity during the establishment of the initial infection of the liver is poorly understood. Here we identify a vector protein by mass spectrometry, with similarity to the human gamma interferon  ...[more]

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