Thermal proteome profiling of Vibrio mutants exposed to arsenate
Ontology highlight
ABSTRACT: We performed two dimensional thermal proteome profiling (2D-TPP) of deletion mutants of Vibrio cholerae to study their function upon exposure to arsenate.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Vibrio Cholerae Serotype O1 (strain Atcc 39315 / El Tor Inaba N16961)
Project description:We performed two dimensional thermal proteome profiling (2D-TPP) of D-Arginine in Vibrio cholerae to study the proteins that it binds to.
Project description:We used thermal proteome profiling (TPP) to study the thermostability of Clostridium saccharolyticum in the presence of duloxetine.
Project description:We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
Project description:We performed thermal proteome profiling (TPP) to study changes to protein thermal stability and abundance of CA-074me (cathepsin inhibitor) in RAW264.7 macrophages after Salmonella typhimurium infection.
Project description:We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.