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The effects of silencing PTX3 oin the proteome of human endothelial cells

ABSTRACT: The human long pentraxin PTX3 has complex regulatory roles at the crossroad of innate immunity, inflammation, and tissue repair. PTX3 can be produced by various cell types, including vascular endothelial cells (ECs), in response to pro-inflammatory cytokines or bacterial molecules. But PTX3 has also been involved in the regulation of cardiovascular biology, even if contrasting results have been so far provided in both preclinical and clinical research. Therefore, we compared the proteomic profiles of human ECs isolated from umbilical cords (Human Umbilical Vein ECs, HUVECs), and we identified 19 proteins more abundant in the proteome of control ECs and 23 proteins more expressed in PTX3 silenced cells.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Maura Brioschi

LAB HEAD: Cristina Banfi

PROVIDER: PXD036691 | Pride | 2023-03-11

REPOSITORIES: Pride

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The Effects of Silencing PTX3 on the Proteome of Human Endothelial Cells.

Banfi Cristina C Brioschi Maura M Vicentini Lucia M LM Cattaneo Maria Grazia MG

International journal of molecular sciences 20221103 21

The human long pentraxin PTX3 has complex regulatory roles at the crossroad of innate immunity, inflammation, and tissue repair. PTX3 can be produced by various cell types, including vascular endothelial cells (ECs), in response to pro-inflammatory cytokines or bacterial molecules. PTX3 has also been involved in the regulation of cardiovascular biology, even if ambiguous results have been so far provided in both preclinical and clinical research. In this study, we compared the proteomic profiles ...[more]

PMID: 36362273

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