Proteomics

Dataset Information

0

The effects of silencing PCYOX1 on the proteome of mitochondria isolated from murine cardiomyocites HL-1

ABSTRACT: In order to evaluate the impact of PCYOX1 deletion on cardiomyocytes, we applied a label-free mass spectrometry-based proteomic approach to compare the proteome of mitochondria from Hl-1 after PCYOX1 gene silencing. Pcyox1 was silenced using shRNA lentiviral particles. Control cells were treated with shRNA lentiviral particles containing a negative construct.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Erica Gianazza  

LAB HEAD: Cristina Banfi

PROVIDER: PXD040093 | Pride | 2026-04-27

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
HL1_shPCYOX_mito.DerivedFromSearch8298afd7-114d-426c-80c5-9f8668352697.mgf Mgf
HL1_shPCYOX_mito.mzid.gz Mzid
HL1mito1_neg_UDMSE_01.mzML Mzml
HL1mito1_neg_UDMSE_02.mzML Mzml
HL1mito1_neg_UDMSE_03.mzML Mzml
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Publications

Prenylcysteine Oxidase 1 Deficiency Protects the Cardiac Muscle Cell Line HL-1 Against Ischaemic/Hypoxic Stress.

Banfi Cristina C   Brocca Lisa L   Bascucci Alice A   Papaianni Giulia Giusy GG   Mallia Alice A   Eligini Sonia S  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20260401 8

Loss of cardiomyocytes during hypoxia-reoxygenation injury contributes to adverse myocardial remodeling, resulting in hypertrophy of surviving cardiomyocytes, interstitial fibrosis, and ultimately, heart dysfunction. Despite extensive research in the field, there is currently no specific treatment available for myocardial hypoxia-reoxygenation injury to prevent cardiomyocyte death. Prenylcysteine oxidase 1 (PCYOX1) is a pro-oxidant, FAD-dependent thioether oxidase that generates hydrogen peroxid  ...[more]