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Combined transcriptomic and proteomic analysis of chicken DF-1 cells following innate immune stimulations

ABSTRACT: The goal of our study was to experimentally quantify both at the transcriptomic and proteomic level the effects of comprehensive innate immune stimulations in chicken. To this end, SILAC-labeled chicken DF-1 cells were mock-treated or treated with either ChIFN1 (1000 IU/mL for 6h), transfected poly (I:C) (4,25 ug/mL for 6 h) or infected with Infectious Bursal Disease Virus strain PBG-98 (IBDV, MOI 5 for 8h). Total cellular RNA was extracted using RNeasy Mini Kit (QIAGEN) and Illumina libraries were prepared and sequenced. In parallel, proteins were extracted and processed for SILAC-based quantitative mass-spectrometry. Samples were run on a LTQ-Velos Orbitrap tandem mass spectrometer and the data processing and quantification were performed by the MaxQuant software.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Frederic Sorgeloos

LAB HEAD: Ian Goodfellow

PROVIDER: PXD057686 | Pride | 2026-05-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	GCF_000002315.6_GRCg6a_protein.fasta	Fasta
	IBDV-PBG98-mqpar.xml	Xml
	IBDV-PBG98-proteinGroups.txt	Txt
	IFN-mqpar.xml	Xml
	IFN-proteinGroups.txt	Txt

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