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Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein - DIA cell fractions

ABSTRACT: Loss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Hypothalamus Cell

SUBMITTER: Oded Kleifeld

LAB HEAD: Philippa Melamed

PROVIDER: PXD065908 | Pride | 2026-05-14

REPOSITORIES: Pride

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			Action	DRS
	2024-07-01-decoys-reviewed-contam-UP000000589.fas	Other
	Cyto.zip	Other
	Fraction_Info.txt	Txt
	Mito.zip	Other
	Pre12K.zip	Other

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Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein -Total

Project description:Loss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides. We tetsted the changes in the whole proteome upon Mkrn3 overexpression

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Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein - Mkrn3-AP-MS

Project description:Loss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides. This is work is for the affinity purification mass spectrometry (AP-MS)of MKRN3

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RNA binding activity of Mkrn3 directs translation and cellular distribuition of the Gnrh1 precurser protein away from the the site of its processing to the mature GnRH peptide

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