Mus musculus
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR087273.fastq.gz | Fastqsanger.gz | |||
| DRR087274.fastq.gz | Fastqsanger.gz | |||
| DRR087275.fastq.gz | Fastqsanger.gz | |||
| DRR087276.fastq.gz | Fastqsanger.gz | |||
| DRR087277.fastq.gz | Fastqsanger.gz |
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Project description:Intracellular accumulation of a-synuclein (a-syn) and formation of Lewy bodies are neuropathological characteristics of Parkinson‘s disease (PD), related a-synucleinopathies, and other neurodegenerative diseases. Recent evidence suggests that oligomerization and spreading of a-syn from neuron to neuron are key events contributing to the development of PD. To directly visualize and characterize a-syn oligomerization and spreading in vivo, we generated two independent conditional transgenic mouse models based on a-syn protein complementation assays using both split Gaussia luciferase and split Venus YFP. These inducible, neuron-specific transgenic mice allow to directly assess the quantity and subcellular distribution of a-syn oligomers in vivo. Using these innovative mouse models we demonstrate an age dependent accumulation of a specific subtype of a-syn oligomers and their synaptic localization in vivo. We provide in vivo evidence that although a-syn is found throughout neurons a-syn oligomerization takes place at the presynapse. Furthermore, our new mouse models provide strong evidence for a long distance transsynaptic cell-to-cell transfer of de novo generated a-syn oligomers in vivo.
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