Project description:Human iPSC-derived skin organoids provide a powerful model for studying skin development and disease. Here, we investigated the effects of TGF-β treatment on human iPSC-derived skin organoids using single-cell RNA sequencing (scRNA-seq). Transcriptomic analysis identified diverse cellular populations, including keratinocytes and fibroblast-like cells, and revealed significant TGF-β-induced changes in cell states and gene expression. TGF-β treatment upregulated profibrotic and extracellular matrix-associated genes and activated pathways related to tissue remodeling and epithelial–mesenchymal transition. These findings demonstrate that iPSC-derived skin organoids recapitulate key features of TGF-β-mediated skin remodeling and provide a useful platform for studying fibrosis and cellular heterogeneity at single-cell resolution.
Project description:Scleroderma is a chronic connective tissue disease characterized by skin thickening and plaque morphea, as well as hard, shiny skin. Serious involvement is observed from the superficial dermis down to the fascia and muscle. However, this condition is difficult to treat and evaluate. In this study, induced pluripotent stem cell (iPSC)-derived 3D epithelial and mesenchymal organoids were used for localized scleroderma therapy.
Project description:Millions suffer from skin diseases. Functional epidermal stem cells are needed in skin therapy or drug screening in vitro. We obtained functional epidermal stem cells with intact stemness and cell junctions by treating them with wnt3a. Moreover, epidermal stem cell-derived exosomes were useful in epidermal development. Finally, functional epidermal 3D organoids with polarity were cultured using wnt3a and the supernatant derived from epidermal stem cells and fresh medium in a 1:1 ratio. These results provide novel directions for the improvement of skin organoids and their potential in clinical application.
Project description:LncRNA and mRNA profiling of human iPSC derived cerebral organoids (Propofol treated vs. DMSO control) were determined. LncRNA and mRNA profiling of human iPSC derived cerebral organoids (Propofol treated vs. DMSO control) were determined.
Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of Forkhead Box P1, in human iPSC-derived forebrain organoids with and without FOXP1. We generated cell type-specific gene expression profiles of FOXP1 in progenitors and excitatory neurons of dorsal telencephalic lineage.
Project description:There is a significant demand for intermediate-scale bioreactors in academic and industrial institutions to produce cells for various applications in drug screening and/or cell therapy. However, the application of these bioreactors in cultivating hiPSC-derived immune cells and other blood cells is noticeably lacking. To address this gap, we have developed a xeno-free and chemically defined intermediate-scale bioreactor platform, which allows for the generation of standardized human iPSC-derived hematopoietic organoids and subsequent continuous production of macrophages (iPSC-Mac).
Project description:Induced pluripotent stem cells (iPSCs) are a promising resource for allogeneic cartilage transplantation to treat articular cartilage defects that do not heal spontaneously and often progress to debilitating conditions, such as osteoarthritis. However, to the best of our knowledge, allogeneic cartilage transplantation into primate models has never been assessed. Here, we show that allogeneic iPSC-derived cartilage organoids survive and integrate as well as function as articular cartilage in a primate model of chondral defects in the knee joints. Histological analysis revealed that allogeneic iPSC-derived cartilage organoids in chondral defects elicited no immune reaction and directly contributed to the tissue repair for at least four months. iPSC-derived cartilage organoids integrated with the host native articular cartilage and prevented degeneration of the surrounding cartilage. Single-cell RNA-sequence analysis indicated that iPSC-derived cartilage organoids differentiated after transplantation, acquiring expression of PRG4 that is crucial for joint lubrication. Pathway analysis suggested the involvement of SIK3 inactivation, verified through molecular experiments. Our study outcomes suggest that allogeneic transplantation of iPSC-derived cartilage organoids may be clinically applicable for the treatment of patients with chondral defects of the articular cartilage.
