Project description:The transcription factor Snail has been proposed to mediate epithelial-to-mesenchymal transition (EMT) and confer mesenchymal invasive phenotype to epithelial cancer cells To analyze the molecular effects of ectopic Snail expression on an epithelial breast cancer cell line, gene expression profiles of MCF-7 cells transfected to overexpress Snail-6SA variant (MCF-7-Snail) and MCF-7 cells transfected with control plasmid (MCF-7-control) were compared. Development of the cell lines has been previously reported by Zhou et al. (PMID: 15448698).
Project description:The transcription factor Snail has been proposed to mediate epithelial-to-mesenchymal transition (EMT) and confer mesenchymal invasive phenotype to epithelial cancer cells To analyze the molecular effects of ectopic Snail expression on an epithelial breast cancer cell line, gene expression profiles of MCF-7 cells transfected to overexpress Snail-6SA variant (MCF-7-Snail) and MCF-7 cells transfected with control plasmid (MCF-7-control) were compared. Development of the cell lines has been previously reported by Zhou et al. (PMID: 15448698). Dataset includes 3 replicate cultures of MCF-7-Snail cells and 3 replicate cultures of MCF-7-control cells
Project description:Pokmeon is an oncogenic transcription factor involved in cell growth, differentiation, and oncogenesis,but its regulatory network in breast cancer cells remians elusive . We used microarray to determine the down stream target genes of pokemon by using the MCF-7 cell line with stable ectopic expression of pokemon. The MCF-7 cell clones with high ectopic expression of pokemon were screened by the Hygromycin B,and the control cell line was also obtained by introducing empty vector .Both the RNA were extracted and hybridized with 22k cDNA spots from CapitalBio, Chin
Project description:Pokmeon is an oncogenic transcription factor involved in cell growth, differentiation, and oncogenesis,but its regulatory network in breast cancer cells remians elusive . We used microarray to determine the down stream target genes of pokemon by using the MCF-7 cell line with stable ectopic expression of pokemon.
Project description:Gene expression profiling to determine transcriptome changes following Snail or Slug expression in MCF-7 breast cancer cells Samples were isolated in three biological replicates for microarray analysis. Four MCF-7 breast cancer cell subsets: MCF-7(untreated), MCF-7 (with Control adenovirus), MCF-7 (with Snail adenovirus) and MCF-7 (with Slug adenovirus). RNA was purified on Day 0 for untreated, and on days 1,2 and 4 for the remaining three subsets for gene expression profiling on microarray.
Project description:The human breast cancer cell line MCF7 was trasnduced with SNAIL trasncription factor. In-depth proteomic analysis revealed several important cellular processes regulated, especially related to epigenetic control of cell proliferation and protein expression.
Project description:We have applied our research on a well-documented biological model, the epithelial cell evasion mediated by the ectopic expression of the snail protein. Indeed, ectopic expression of Snail, a transcriptional repressor, is widely used to study the initial step of epithelial cell evasion also called Epithelial to Mesenchymal Transition (EMT). During this process, cells loose their intercellular junctions and acquired motility and an invasive behaviour. In order to have a temporal view of the gene expression modulation that occurred during EMT, we have established a biological model based on the conditional expression of snail protein in human non-invasive MCF-7 breast carcinoma cells that do not express endogenous snail and exhibit a well-organized epithelial phenotype. 96 hours after snail induction, the epithelial to mesenchymal cell phenotype conversion is completed.
Project description:Gene expression profiling to determine transcriptome changes following Snail or Slug expression in MCF-7 breast cancer cells Samples were isolated in three biological replicates for microarray analysis.
Project description:Identifying PDEF regulated genes may shed light on the mechanism by which PDEF may induce breast cancer progression. To that purpose, we have used the MCF-7 human breast tumor cell line model to identify PDEF induced genes. Briefly, PDEF expression was down regulated by shRNA in MCF-7 cells and RNA probes from PDEF-down regulated and control MCF-7 cells were used to screen the Affymetrics HG-U133A Gene Chips. This analysis found 62 genes that were induced 2-fold or higher by PDEF. Further analysis of 3 of these genes namely S100A7, CEACAM6 and B7-H4 in primary breast tumors showed CEACAM6 as a frequently elevated and co-exressed gene with PDEF in these tumors. We previously reported a role for PDEF (prostate derived Ets transcription factor) in breast tumor progression and its association with poor clinical outcome in ER+ breast cancer. To gain further insights into PDEF action in breast cancer, we down regulated PDEF expression by shRNA in MCF-7 human breast tumor cell line, and screened the HG-U133A human gene chips with probes from PDEF down-regulated and control MCF-7 cells. This analysis identified CEACAM6 as one of the genes induced by PDEF. Further analysis of CEACAM6 expression in relation to PDEF in 93 ER+ primary breast tumors showed largely concordant expression of these molecules. To our knowledge, our findings of CEACAM6 as a PDEF induced gene and their elevated co-expression in breast cancer have not been described before. Data from one replicate experiment is included as a representative example of the data obtained. HG-U133A gene chip pairs were screeened with biotinylated RNA probes from PDEF-down regulated MCF-7 cells (experimental) or from control MCF-7 cells.
