Project description:WWP2 is a E3 ligase which regulates multiple cellular events, such as stress response, differentiation. WWP2 played physiological role in the distal tubules of the kidneys. Whether WWP2 regulated pathophysiological rol in the proximal tubules is unclear. In the present study, we stably overexpressed WWP2 using lenti-viral infection in the human tubular epithelial cells, HK-2, and performed proteinomics analysis to explore WWP2's potential substrates.
Project description:Renal tubular epithelial injury plays a critical role in the progression of renal interstitial fibrosis. In this study, bulk RNA-seq was performed to investigate transcriptomic alterations in human HK-2 proximal tubular epithelial cells following target gene knockdown via sgRNA. Cells transfected with non-targeting sgRNA were used as negative control. Three biological replicates were included for each group. This dataset provides transcriptome resources to uncover molecular events regulated by the target gene in renal tubular epithelial cells.
Project description:Shotgun time-series proteomic analysis based on stable isotope dimethyl labeling and UHPLC-MS/MSC to relatively quantify the changes in protein expression of human proximal tubular HK-2 cells exposed to a diabetic-like microenvironment
Project description:We first performed miRNA analysis on SU-4 cells in suspension, and after 24 and 48 h of HK co-culture. To ensure that no HK cell contamination occurred, SU-4 cells in suspension and after co-culture were purified by CD19-positive magnetic selection, followed by total RNA isolation. Two-condition experiment, S4 vs. S4+HK cells. Biological replicates: 2 suspension controls, 2 co-cultured with HK cells, independently grown and harvested. One replicate per array.
Project description:PRCC-TFE3 is an oncogenic chimeric transcription factor identified in human TFE3-rearranged renal cell carcinoma. To analyze gene expression changes driven by PRCC-TFE3, we generated and utilized an HA-tagged PRCC-TFE3-inducible HK-2 cell line, which expresses HA-tagged PRCC-TFE3 in a doxycycline-dependent manner. Cells were cultured with or without doxycycline, and comprehensive gene expression analysis was conducted using RNA-seq.
Project description:Atlas Human Stress Arrays with 234 stress-related genes immobilised on nylon membranes (Clontech, UK) were used to identify the changes in mRNA expression in human proximal tubular HK-2 cells in response to HSA overload or AngII. Keywords: Stress response
Project description:This study aims to elucidate differential protein expression in HK-2 cells exposed to sodium oxalate and sodium selenite using 4D label free proteomics.