Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of binding sites of the Six1 transcription factor in mouse primary myoblasts and myotubes

ABSTRACT: Identification of binding sites of the Six1 transcription factor in mouse primary myoblasts and myotubes

PROVIDER: PRJNA734569 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14711117.fastq.gz	Fastqsanger.gz
	SRR14711118.fastq.gz	Fastqsanger.gz
	SRR14711119.fastq.gz	Fastqsanger.gz
	SRR14711120.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Identification of binding sites of the Six1 transcription factor in mouse primary myoblasts and myotubes

Project description:Binding sites of the Six1 transcription factor were identified in primary myoblasts and myotubes of mice using ChIP-sequencing

2021-12-01 | GSE175999 | GEO

Genomic mapping of Six1 binding sites during skeletal myoblast differentiation

Project description:In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 3 different timepoints to perform ChIP-on-Chip of Six1, which is a key muscle regulator. We identified global loci bound by Six1 during skeletal myoblast differentiation. C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at three timepoints were harvested for ChIP-on-Chip, including myoblasts stage, 24h after differentiation and myotubes (96h after differentiation). Myotubes were detached from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates were used for each time point experiment. A microarray set counts 3 arrays (Custom Arrays A, B and C) for a total of approximately 2.9 million probes.

2010-07-05 | E-GEOD-20150 | biostudies-arrayexpress

Genome-wide maps of c-Myc binding sites in myoblasts and myotubes

Project description:Genome-wide maps of c-Myc binding sites in myoblasts and myotubes

| PRJNA430194 | ENA

circRNA expression in C2C12 myoblasts and myotubes

Project description:To determine the circRNA expression profile in C2C12 myoblasts and myotubes, we used mouse circRNA microarray from Arraystar to examine the expression of circRNAs in C2C12 myoblasts and myotubes.

2021-03-19 | GSE112014 | GEO

lncRNA expression in C2C12 myoblasts and myotubes

Project description:To determine the lncRNA expression profile in C2C12 myoblasts and myotubes, we used mouse lncRNA microarray from Arraystar to examine the expression of lncRNAs in C2C12 myoblasts and myotubes.

2020-02-18 | GSE101499 | GEO

Genomic mapping of Six1 binding sites during skeletal myoblast differentiation

2010-07-05 | GSE20150 | GEO

Genome-wide maps of c-Myc binding sites in myoblasts and myotubes

Project description:By applying ChIP-seq, we generated genome-wide maps of c-Myc in skeletal myoblasts and myotubes. We found that c-Myc binds to 19354 confident target with a large portion residing in the intergenic regions. In addition, c-Myc was found to regulate many loci. Further detailed study revealed that c-Myc can regulate some genes, miRNAs, and lincRNAs which are fucntional in skeletal myogenesis. In this study, we identified a c-Myc induced regulatory pathway in myogensis.

2018-01-17 | GSE109223 | GEO

Transcriptome changes during the differentiation of myoblasts into myotubes

Project description:This study aimed to interrogate the interrelationship between 3D genome organization and global gene expression during muscle development using a mouse C2C12 cell line as an in vitro model. The C2C12 cell line is a well-established and extensively studied in vitro model derived from serial passage of myoblasts cultured from the thigh muscle of C3H mice after a crush injury. C2C12 cells divide when mitogens are present in the culture medium and spontaneously differentiate into muscle-like multinucleated (myotubes) cells if the medium is depleted of mitogens (i.e. serum; (Bischoff 1986)). C2C12 cells were either harvested as: 1) proliferating myoblasts (Myoblasts); 2) myotubes that were not treated with AraC (as such these myotubes contained myoblasts) - Myotubes(Day3); or 3) myotubes which were treated with AraC (myoblasts were largely depleted from these myotube cultures; Myotubes(Day7+AraC).

2017-04-07 | GSE84158 | GEO

Genome-wide maps of Sin3A and Sin3B binding in C2C12 myoblasts and differentiated myotubes

Project description:Sin3A and Sin3B bind to distinct and overlapping target sites. Sin3 proteins bind to distinct sites in cycling myoblasts whereas there is a converge of Sin3A and Sin3B proteins to sites in differentiated myotubes. We identified a subset of Sin3 target genes involved in muscle differentiation and physiology and showed that conditional ablation of Sin3 proteins in vivo in mouse results in sarcomere defects

2011-01-01 | GSE22554 | GEO

Gene expression induced by TSA in undifferentiated human primary myoblasts versus terminally differentiated human myotubes

Project description:Gene expression analysis in human skeletal myoblasts (undifferentiated mononucleated cells, cultured in growth medium - 15% FBS) and human skeletal myotubes (pre-formed myotubes, cultured in differentiation medium – 2% horse serum) exposed to the HDACi TSA (50nM) for 24 hours TSA treated undifferentiated human primary myoblasts and terminally differentiated human myotubes

2011-03-01 | E-GEOD-27073 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data