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TranscriptomicsChanges in gene expression induced by CDK9 inhibition alone and in combination with fludarabine
Using a transcriptomics approach we explored the mechanism(s) of synergy observed between CDKI-73 and fludarabine in primary CLL cells. The cytotoxic effects of CDKI-73 were associated with transcriptional inhibition of cdk9 target genes including MCL1 and XIAP. In contrast, fludarabine induced the ...
ORGANISM(S): Homo sapiens 
2013-06-25 | E-GEOD-48258 | biostudies-arrayexpress
TranscriptomicsExpression profiling of HeLa cell line treated with CDK9 inhibitor
CDK9 is the kinase subunit of P-TEFb that enables RNA polymerase (Pol) II to transit from promoter-proximal pausing to productive elongation. Although considerable interest exists in CDK9 as a therapeutic target, little progress has been made due to the lack of highly selective inhibitors. Here, we ...
ORGANISM(S): Homo sapiens 
2015-06-17 | E-GEOD-60952 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of Cdk9-dependence for VSV-induced genes in Drosophila cells
To determine the Cdk9 targets of VSV-induced genes in Drosophila cells at 4 hours post-infection Drosophila cells were treated with Bgal or Cdk9 and infected with VSV (MOI 10) for 4 hours, in two independent biological replicates. Total RNA was isolated using Trizol and microarray experiments were p...
ORGANISM(S): Drosophila melanogaster 
2012-10-18 | E-GEOD-41242 | biostudies-arrayexpress
GenomicsCompensatory induction of MYC expression by sustained CDK9 inhibition via a BRD4-dependent mechanism: Pol II occupancy profiling by ChIP-Seq in HeLa cell line in the presence or absence of CDK9 inhibition
CDK9 is the kinase subunit of P-TEFb that enables RNA polymerase (Pol) II to transit from promoter-proximal pausing to productive elongation. Although considerable interest exists in CDK9 as a therapeutic target, little progress has been made due to the lack of highly selective inhibitors. Here, we ...
ORGANISM(S): Homo sapiens 
2015-06-17 | E-GEOD-60953 | biostudies-arrayexpress
GenomicsChIPseq to profile CDK9 and Hes1 occupancy in bone marrow-derived macrophages from C57BL/6 mice
We characterized the CDK9 and Hes1 occupancy on gene loci in conditions of unstimuated and LPS stimualtion in BMDMs BMDMs were left untreated or stimulated with LPS for 1 hour. CDK9 or Hes1 ChIP was performed and the DNA products were subject to ChIPseq
ORGANISM(S): Mus musculus 
2016-06-13 | E-GEOD-77334 | biostudies-arrayexpress
ProteomicsCardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodeling
Hypertensive heart failure highlights an urgent need for effective therapeutic strategies. Protein kinases regulate multiple pathways in cardiac pathophysiology and may provide promising therapeutic targets. Here, we identified a Cyclin-dependent kinase, CDK9, promoting inflammation and cardiac remo...
ORGANISM(S): Rattus norvegicus (Rat) 
2026-06-15 | PXD074483 | Pride
Angiotensin ii  cdk9  nf-κb p65  cardiac remodeling  cardiomyocytes 
ProteomicsCDK9 interacts with a RanGTP-NEMP1-Importin-β complex to regulate erythroid enucleation
Erythroid enucleation is the final stage of erythroid terminal differentiation and involves the separation of an orthochromatic erythroblast into two daughter cells; a pyrenocyte containing the extruded nucleus, and a reticulocyte that will become a red blood cell. Our previous work identified CDK9 ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-01 | PXD072077 | Pride
Enucleation Cdk9 Erythropoiesis Importin-beta 
TranscriptomicsGlobal Run-On sequencing (GRO-seq) of HeLa cells under CDK9 inhibitor tratment to study elongation checkpoints at both ends of RNA polymerase II-transcribed genes.
Global Run-On sequencing (GRO-seq) was carried out on nuclei isolated from HeLa cells after 30 minutes of treatment with CDK9 inhibitors KM05382 and DRB and from two untreated controls. Libraries of nascent RNAs were generated and sequenced using Illumina Hi-seq technology. GRO-seq reads were proces...
ORGANISM(S): Homo sapiens 
2015-02-26 | E-MTAB-3360 | biostudies-arrayexpress
ProteomicsIdentification of CDK9 targets using DRB and quantitative phosphoproteomics
CDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II) in higher eukaryotes. Phosphorylation of targets including the elongation factor SPT5 and the carboxyl-terminal domain (CTD) of RNA pol II allow the polymerase to pass an early e...
ORGANISM(S): Homo sapiens (Human) 
2022-08-22 | PXD026720 | Pride
Hela Silac Drb Phosphoproteomics Cdk9 
ProteomicsProteomic characterization of NHL cells in presence of CDK9 inhibitor
non-Hodgkins lymphoma cells were treated with a CDK9 inhibitor to determine which proteins and phosphorylation events are affected by CDK9 in lymphoma.
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD035858 | Pride
Lymphoma Phosphoproteomics Mass spectrometry Cdk9 
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