Mitochondria constantly undergo fusion and fission events, referred as mitochondrial dynamics, which determine intracellular mitochondrial architecture and bioenergetics. Cultured cell studies demonstrate that mitochondrial dynamics are acutely regulated by phosphorylation of the mitochondrial fissi...
In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well as cardiovascular diseases and cancer. Here, we developed n...
Mass spectrometry of GST-tagged DRP1 expressed in HEK cells either alone or co-expressed with WT DLK or DLK S302A (Kinase dead mutant).
("D" DRP1 only; "D-D" DRP1 + DLK WT; "D-S" DRP1 + DLK S302A)
The presence of redox-active molecules containing catenated sulfur atoms (supersulfide) in living organisms has led to a review of the concepts of redox biology and its translational strategy. Glutathione (GSH) is the body’s primary detoxifier and antioxidant, and its oxidized form (GSSG) has been c...
Drp1 is a GTPase involves in mitochondrial division. The phosphorylation of Drp1 is reported to influence Drp1 activity. Two well known Drp1 phosphorylation sites have been identified. In here, we in vitro phosphorylate S579 site by ERK2 kinase on WT-Drp1 or S600D-Drp1 mutant and try to identify the...
This study demonstrated that circadian gene clock plays an important role in the regulation of mitochondrial homeostasis. In this process, CLOCK interacts with PUF60 and affects the mRNA stability of Drp1.
NME3 is a member of the nucleoside diphosphate kinase (NDPK) family localized on the mitochondrial outer membrane (MOM). Here, we report a role of NME3 in hypoxia-induced mitophagy dependent on its active site phosphohistidine but not the NDPK function. Mice carrying a knock-in mutation in the Nme3 ...
