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ProteomicsQuantitative evaluation of FASP and MED FASP protocols
In this work we re-evaluate FASP and the related Multienzyme digestion (MED)-FASP method. We use different types of animal tissue and cultured cells, and test the performance of the method under various conditions. We analyze the protein-to-peptide conversion by assessing the yield of peptides, freq...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-04-28 | PXD003733 | Pride
Fasp  med fasp  missing cleavages  reproducibility 
ProteomicsOptimization of quantitative proteomic analysis of clots
The aim of our study was to optimize quantitative proteomic analysis of fibrin clots prepared ex vivo from citrated plasma of the peripheral blood drawn from patients with prior venous thromboembolism. We used a multiple enzyme digestion filter aided sample preparation (MED FASP) method combined wi...
ORGANISM(S): Homo sapiens (Human) 
2017-12-08 | PXD006814 | Pride
Proteomics Venous thromboembolism Clot Med fasp 
ProteomicsComparison of proteome composition of serum enriched in extracellular vesicles isolated from polycythemia vera patients and healthy controls
Extracellular vesicles (EVs), e.g., exosomes and microvesicles, are one of the main networks of intercellular communication. In myeloproliferative disorders, such as polycythemia vera (PV), excess of EVs originating from overabundant blood cells can directly contribute to thrombosis through their pr...
ORGANISM(S): Homo sapiens (Human) 
2019-05-13 | PXD013234 | Pride
Human plasma Extracellular vesicles Vesicles 
ProteomicsQuantitative Analysis of of Human Liver
Large scale proteomic analysis of human liver from 10 donors
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD003458 | Pride
Human liver  total protein approach  med-fasp  fasp 
UnknownOptimization of quantitative proteomic analysis of clots generated from plasma of patients with venous thromboembolism.
Not available
S-EPMC5706328 | biostudies-literature
UnknownQuantitative analysis of the Escherichia coli proteome.
Not available
S-EPMC4459560 | biostudies-literature
ProteomicsPlasma fibrin clot-bound proteins in antiphospholipid syndrome and venous thromboembolism: relation with inflammation and thrombosis
Antiphospholipid syndrome (APS) is associated with arterial and venous thrombosis. The unfavorable fibrin clot phenotype, including formation of dense and poorly lysable clots, has been reported both in thrombotic APS and venous thromboembolism (VTE). The presence and amount of different proteins wi...
ORGANISM(S): Homo sapiens (Human) 
2019-08-20 | PXD008434 | Pride
Antiphospholipid syndrome Thrombosis Venous thromboembolism Inflammation 
ProteomicsComparative analysis of mouse muscles types
The dataset presents FASP- and LC-MS/MS-based analysis of whole lysates of tree types of mouse muscle tissue: white and red muscle fibers and heart muscle. The measurements were performed using five biological replicates for each tissue type. The dataset cover 7200 proteins. The data were used for ...
ORGANISM(S): Mus musculus (Mouse) 
2016-06-21 | PXD002152 | Pride
Skeletal muscle proteome Metabolism Quantitative proteomics Protein titer 'total protein approach' Glycolysis Heart muscle proteome Med fasp 
UnknownComparison of Proteome Composition of Serum Enriched in Extracellular Vesicles Isolated from Polycythemia Vera Patients and Healthy Controls.
Not available
S-EPMC6631625 | biostudies-literature
Proteomics‘Shotgun’ Proteome Analyses Do Not Require Alkylation of Cysteine
It is a common belief that reduction of disulfide bridges and alkylation of thiols in proteins are indispensable steps in proteomic sample preparation. Since this chemical procedure is often incomplete and prone to side reactions and can results in various artefacts we reexamined its importance. We ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-21 | PXD014000 | Pride
Thiol alkylation Iodoacetamide Shotgun proteomics 
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