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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsPulse SILAC analysis of mitochondrial complex assembly
Pulse SILAC analysis of mitochondrial protein complex assembly Pulse times (hours): P3, P4, P6, P12 Bogenhagen et al. Cell Reports 2018
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-03 | MSV000084749 | MassIVE
ProteomicsAnalysis of newly synthesized proteins more sensitive to Proteinase K using pulse SILAC and control sample for pulse SILAC experiment in which yeast cells were heat shock for 15 min and supernatant fractions analyzed (Experiment 1)
Accurate and efficient folding of nascent protein sequences into their native state requires support from the protein homeostasis network. Herein we probed which newly translated proteins are thermo-sensitive to infer which polypeptides require more time to fold within the proteome. Specifically, we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-10 | PXD028934 | Pride
Protein homeostasis Yeast Pulse silac Limited proteolysis 
ProteomicsPulse SILAC analysis of immuno-precipitated mitochondrial complexes I, IV and V
Pulse SILAC analysis of mitochondrial complexes I, IV and V HeLa cells
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-03 | MSV000084748 | MassIVE
ProteomicsManassantin A SILAC-Pulse Proteolysis
Manassantin A is a natural product that has been isolated from the perennial herb Saururus chinensis Baill and the aquatic plant Saururus cernuus. Manassantin A has been shown to possess potent hypoxia inducible factor 1 alpha (HIF-1α) inhibitory activity in a cell-based assay screen of thousands o...
ORGANISM(S): Homo sapiens (Human) 
2016-07-19 | PXD002305 | Pride
Drug mode-of-action In-gel digestion Mda-mb-231 Pulse proteolysis Stable isotope labeling of amino acids in cell culture Manassantin a 
ProteomicsIdentification of protein substrates of the Cullin-RING E3 ubiquitin ligase CRL4 through its substrate recognition factors DCAFs
Cullin-RING finger Ligases (CRLs) represent the largest family of E3 ubiquitin ligases and are responsible for ubiquitination of ~20% of cellular proteins degraded through the proteasome, catalyzing the transfer of E2-loaded ubiquitin to a substrate. Eight Cullins were described in vertebrates and a...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD037482 | Pride
Ubiquitylation Pulse-silac Dcafs Bioid Ddb1 Interactome Cullin4 
ProteomicsPulse-SILAC and interactomics reveal distinct DDB1-CUL4 associated factors (DCAFs), cellular functions, and protein substrates
Cullin-RING finger ligases (CRLs) represent the largest family of ubiquitin ligases. They are responsible for the ubiquitination of ~20% of cellular proteins degraded through the proteasome, by catalyzing the transfer of E2-loaded ubiquitin to a substrate. Seven Cullins are described in vertebrates....
ORGANISM(S): Homo sapiens (Human) 
2023-10-11 | PXD042903 | Pride
Pulse-silac Dcafs Ubiquitination Bioid Ddb1 Interactome Cullin4 
ProteomicsPulse-chase SILAC of Mycobacterium tuberculosis infected THP-1 macrophages
Pulse chase SILAC was used to identify protein turnover within human macrophages infected with mycobacterium tuberculosis CDC1551, a ppe38-71 mutant strain, a complemented strain and an uninfected control.
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD021166 | Pride
Lc-ms/ms Tuberculosis Silac Macrophages 
ProteomicsProtein and peptide turnover measurements using pulsed SILAC-TMT approach
We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved in...
ORGANISM(S): Homo sapiens (Human) 
2018-02-09 | PXD008579 | Pride
Degradation rates Protein turnover Proteoforms Posttranslational cleavage Synthesis rates Tmt Splice variants Pulsed silac Posttranslational modification 
ProteomicsA novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.
We developed a pulse-chase variant of SILAC (stable isotope labeling by amino acids in cell culture pcSILAC). pcSILAC can quantitate in one experiment and for two conditions the relative levels of proteins newly synthesized in a given time as well as the relative levels of remaining preexisting prot...
ORGANISM(S): Homo sapiens (Human) 
2013-12-10 | PXD000538 | Pride
Hsp90 T-cells Pulse-chase Silac Synthesis rate Decay rate 
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