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ProteomicsThe CRL4B E3 ubiquitin ligase complex regulates mitosis by recruiting phospho-specific DCAFs
The cullin scaffolds CUL4A and CUL4B assemble E3-ligase complexes regulating multiple mostly chromatin-associated cellular functions. Although they are structurally similar, we found that the unique N-terminal extension of CUL4B is heavily phosphorylated during mitosis, and this pattern is perturbed...
ORGANISM(S): Homo sapiens (Human) 
2023-06-15 | PXD035329 | Pride
Mitosis Dcaf E3 ubiquitin ligase Spindle positioning Phosphorylation Cell cortex Cell cycle regulation 
ProteomicsIdentification of protein substrates of the Cullin-RING E3 ubiquitin ligase CRL4 through its substrate recognition factors DCAFs
Cullin-RING finger Ligases (CRLs) represent the largest family of E3 ubiquitin ligases and are responsible for ubiquitination of ~20% of cellular proteins degraded through the proteasome, catalyzing the transfer of E2-loaded ubiquitin to a substrate. Eight Cullins were described in vertebrates and a...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD037482 | Pride
Ubiquitylation Pulse-silac Dcafs Bioid Ddb1 Interactome Cullin4 
UnknownThe CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs.
Not available
S-EPMC10476281 | biostudies-literature
ProteomicsPulse-SILAC and interactomics reveal distinct DDB1-CUL4 associated factors (DCAFs), cellular functions, and protein substrates
Cullin-RING finger ligases (CRLs) represent the largest family of ubiquitin ligases. They are responsible for the ubiquitination of ~20% of cellular proteins degraded through the proteasome, by catalyzing the transfer of E2-loaded ubiquitin to a substrate. Seven Cullins are described in vertebrates....
ORGANISM(S): Homo sapiens (Human) 
2023-10-11 | PXD042903 | Pride
Pulse-silac Dcafs Ubiquitination Bioid Ddb1 Interactome Cullin4 
UnknownThe CUL4B-based E3 ubiquitin ligase regulates mitosis and brain development by recruiting phospho-specific DCAFs
Not available
S-SCDT-10_15252-EMBJ_2022112847 | biostudies-other
ProteomicsProximity biotinylation of HEK293T cells stably expressing AirID-DCAFs
HEK293T cells stably expressing AGIA-AirID-DCAF, -SKP2, and -IκBα were cultured in a 10-cm dish in biological replicates (n = 2) and treated with 10 µM MG132 for 6 h in the presence of 10 µM biotin for 24 h. The cell pellet was then lysed in 300 µL of a guanidine buffer (6 M guanidine-HCl, 100 mM HE...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057875 | JPOST Repository
UnknownIntegrative analysis of multi-omics data reveals distinct impacts of DDB1-CUL4 associated factors in human lung adenocarcinomas.
Not available
S-EPMC5428704 | biostudies-literature
UnknownPulse-SILAC and Interactomics Reveal Distinct DDB1-CUL4-Associated Factors, Cellular Functions, and Protein Substrates.
Not available
S-EPMC10565876 | biostudies-literature
UnknownRNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells.
Not available
S-EPMC3018799 | biostudies-literature
ProteomicsDynamic exchange of CRL4 regulated by CAND1 and CAND2
CAND1 and CAND2 have been established as exhange factors for CRL1s, however, how they regulate the dynamic exchange of CRL4s remains not fully understood. This project aims to study and compare the effect of CAND proteins on the exchange levels of multiple DCAFs, thus providing insights into the fun...
ORGANISM(S): Homo sapiens (Human) 
2026-02-12 | PXD063817 | Pride
Silac  human  cullin-ring ubiquitin ligases 
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