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TranscriptomicsTcRBP40 pull-down ribonomics
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
2012-06-30 | E-MEXP-3057 | biostudies-arrayexpress
ProteomicsRNA pull down experiment for vA-202 (ANAPC1P2) lncRNA
This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
Human Rna pull down Orbutrap ms 
GenomicsRNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
PRJNA561512 | ENA
UnknownProtocol for detecting lncRNA-protein interactions in vitro by tRSA RNA pull-down assay.
Not available
S-EPMC10789616 | biostudies-literature
ProteomicsBiotin-poly-dG or dA pull-down assay
The products of the pull-down assay of mouse brain lysate were analyzed by mass spectrometry.
ORGANISM(S): Mus Musculus (mouse) 
2025-08-18 | PXD058062 | JPOST Repository
ProteomicsEZH2 pre-mRNA pull-down screening for proteins that regulate EZH2 alternative splicing
Post-transcriptional regulation mediated by RNA-binding proteins is essential for B cell development and differentiation. EZH2 is a key regulatory protein involved in B cell development and the germinal center response, and its expression is subject to alternative splicing regulation. Skipping of ex...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-30 | PXD077818 | Pride
Pull down Ezh2 
ProteomicscircMAN1A2_009-pulled down proteins in HeLa cells
CircRNA pulldown assays were conducted to identify proteins interacting with circMAN1A2_009. In summary, HeLa cell protein extracts were incubated with biotin-labeled circMAN1A2_009 probes obtained from Tsingke Biotechnology, China, at room temperature for 1h. Subsequently, streptavidin magnetic bea...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052372 | Pride
Circman1a2_009 Hela Pull-down assays 
TranscriptomicsRNA-biotin based pull down assay with polyA site (PAS) RNA substrates in Hela Nuclear Extract followed by RNA-seq to discover trans-acting RNA molecules involved in mRNA 3 processing
We aimed to discover trans-acting RNA molecules involved in mRNA 3 processing. We reasoned that, if there exist such functional RNAs, they must directly associate with the key machinery responsible for mRNA 3 processing. Therefore, it would be of great value to comprehensively identify RNAs interact...
ORGANISM(S): Homo sapiens 
2017-06-01 | E-MTAB-5383 | biostudies-arrayexpress
ProteomicsProtein interaction partners of drosophila Unr identified by pull down of wild type and an interdomain mutant protein
To identify an RNP interactome of Unr, V5 tagged wildtype protein and a mutant containing several interdomain contacts, that shows reduced protein expression were tansfected in SL2 cells after a siRNA knoch-down of the endogenous protein levels. After pulling down the transfected protein with an ant...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-07-14 | PXD018115 | Pride
Unr Rnp Pull-down Interactome Rbp 
ProteomicsProtein interactome of small RNA fragments: identification and functional implications
Populations of small eukaryotic RNAs, in addition to relatively well recognized molecules (such as miRNAs or siRNAs), also contain fragments derived from all classes of constitutively expressed non-coding RNAs. It has been recently demonstrated that the formation and accumulation of RNA fragments (R...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD010075 | Pride
Protein interactome Rna fragments Rna-protein interaction 
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