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ProteomicsA Targeted in Vivo SILAC Approach for Quantification of Drug Metabolism Enzymes: Regulation by the Constitutive Androstane Receptor
The modulation of drug metabolism enzyme (DME) expression by therapeutic agents is a central mechanism of drug–drug interaction and should be assessed as early as possible in preclinical drug development. Direct measurement of DME levels is typically achieved by Western blotting, qPCR, or microarray...
ORGANISM(S): Mus musculus (Mouse) 
2014-02-14 | PXD000675 | Pride
Drug metabolism  drug−drug interaction  constitutive androstane receptor  protein quantification  targeted in vivo silac 
ProteomicsAn Enhanced in vivo SILAC model for Quantification of Drug Metabolism Enzymes
Many of the enzymes involved in xenobiotic metabolism are maintained at a low basal level and are only synthesized in response to activation of upstream sensor/effector proteins. This induction can have implications in a variety of contexts, particularly during the study of the pharmacokinetics, pha...
ORGANISM(S): Mus musculus (Mouse) 
2015-01-09 | PXD001210 | Pride
In vivo silac Pharmacology Drug-drug interaction Xenobiotic metabolism Drug metabolism Toxicology Cytochrome p450 
UnknownA targeted in vivo SILAC approach for quantification of drug metabolism enzymes: regulation by the constitutive androstane receptor.
Not available
S-EPMC3923450 | biostudies-literature
UnknownAn enhanced in vivo stable isotope labeling by amino acids in cell culture (SILAC) model for quantification of drug metabolism enzymes.
Not available
S-EPMC4349992 | biostudies-literature
ProteomicsPhosphoproteomic analysis reveals cytoskeletal remodelling via CDC42-PAK signaling in lung mucoepidermoid cells upon chronic exposure to cigarette
Despite the fact that smoking is the major cause of lung cancer, there is no clear mechanism detailing how chronic exposure of cigarette smoke alters the global signaling in lung cells. To investigate the altered signaling in lung cells, in this study, we carried out SILAC-based quantitative phospho...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006672 | Pride
Nsclc Smoking In vivo labelling Phosphoproteomics 
ProteomicsPreclinical targeted therapy for retinoblastoma using EpCAM Aptamer siRNA chimeras
siRNAs have played a major role in cancer drug discovery, but their potential is hampered due to off-target effects. Thus, delivery systems like RNA aptamers have been used to enhance the specific delivery of these siRNAs to cancer stem cells. We report the efficacy of three different EpCAM aptamer ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-02 | PXD008221 | Pride
Retinoblastoma Targeted delivery Cancer stem cells Epcam 
ProteomicsVigilin/HDLBP promotes translation of endoplasmic reticulum-targeted mRNAs
Nascent peptide chain targeting to the endoplasmic reticulum (ER) membrane is required for correct localization and efficient translation of membrane-bound and secreted proteins. However, little is known about the contribution of RNA-binding proteins (RBPs) to the recognition, localization and trans...
ORGANISM(S): Homo sapiens (Human) 
2022-05-09 | PXD018316 | Pride
Hdlbp  vigilin  rna-binding protein  kh-domain  endoplasmic reticulum  translation 
ProteomicsMultilayered control of protein turnover by TORC1 and ATG1
To study TORC1-Atg1 signaling comprehensively and to identify potential additional foci of crosstalk, we chose a mass spectrometry (MS)-based phosphoproteomics strategy combing global proteomics screens in vivo with targeted analyses using in vitro kinase reactions. We present the currently largest ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-09-30 | PXD013271 | Pride
Autophagy Yeast Saccharomyces cerevisiae Phosphorylation Torc1 Phosphoproteomics Rapamycin 
ProteomicsEarly response evaluation by single cell signaling profiling in acute myeloid leukemia
Background: A fundamental hallmark of cancer cells is their ability to sustain proliferative signaling and cell survival, reflected in a cellular chemotherapy response that is poorly understood. We questioned whether chemotherapy modulated phospho-signaling at 4 and 24 h in vivo could provide inform...
ORGANISM(S): Homo sapiens (Human) 
2022-10-26 | PXD031916 | Pride
Aml Erk Single cell 
UnknownLarge-Scale and Targeted Quantitative Cross-Linking MS Using Isotope-Labeled Protein Interaction Reporter (PIR) Cross-Linkers.
Not available
S-EPMC5513479 | biostudies-literature
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