The busulfan-treated mouse model showed abnormal testis morphology and reduced sperm number and testis weight. Testicular and sperm damage was most severe at 30 days after busulfan treatment. The protein level of MGAT1 was increased in busulfan-treated mouse testis. The busulfan-treated mouse testes...
Embryonic day 13 (E13), E14, and E16 rat testes and ovaries were used for microarray analysis, as well as E13 testis organ cultures that undergo testis morphogenesis and develop seminiferous cords in vitro. A list of 109 genes resulted from a selective analysis for genes present in male gonadal deve...
The sequence of gene regulatory events that drive neonatal germ cell development in the mammalian testis is not yet clear. We assessed changes in mRNA utilization in the neonatal testis at 1 and 4 dpp, times when the testis contains quiescent gonocytes (1 dpp) and proliferating spermatogonia (4 dpp)...
This project aims at deciphering the human testis proteome and to discover and characterizedunknown proteins in order to better understand the male reproductive physiology.
In order to identify more MPs and get deeper proteomic data of testis. We applied high-pH reverse phase (RP) HPLC to fractionate complex samples.On the other hand, to enhance protein coverage, multi-protease strategies were used for 10% SDS-PAGE short separation samples.
The development of the testis involves a large number of tissue-specific proteins, possibly because that sperms in it are the most divergent of all cell types. Although the proteome of the larval testis of Bombyx mori has been reported, no comprehensive data on adult testis proteome is available for...
The Chromosome-Centric Human Proteome Project (C-HPP) aims at finding existence evidence of missing proteins (MPs) at the early stage. Based on previous research, it has been proved that human testis tissue is suitable for further research of MPs because of its high gene expression level and high tr...
Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination appears to promote an epigenetic reprogramming of the male germ line that is associated with transgenerational adult-onset disease states. Transgenerational effects on the embryonic day 16 (E16) testis demonst...
Spermatogenesis plays an important role in the mammalian testis, involving in the complex processes of mitosis, meiosis, and spermiogenesis. Spermatogenesis may also be disrupted in the absence of the immunological and ‘fence’ functions of the BTB, resulting in male subfertility or infertility. Mice...
A total of 62022 unique peptides were acquired, and 7137 proteins were confidently identified and quantified inthe cryptic (CT) and contralateral (CLT) testis from four unilaterally cryptorchid rams
