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TranscriptomicsTranscription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses
The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 ...
ORGANISM(S): Rattus norvegicus 
2009-04-29 | E-GEOD-5073 | biostudies-arrayexpress
TranscriptomicsExpression Analysis of Trimethyltin Chlorides (TMT) Impact on Embryonic Development
Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to compris...
ORGANISM(S): Danio rerio 
2011-03-23 | E-GEOD-28131 | biostudies-arrayexpress
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
TranscriptomicsMicroarray analysis of the effects of a γ-tocopherol-rich mixture of tocopherols on global gene expression in the prostates of TRAMP mice
We used whole mouse genome microarrays to the effects of γ-TmT on global gene expression in the prostate TRAMP and age-matched C57BL/6 mice were administered either 600 mg/kg of γ-TmT or a control vehicle via oral gavage. Twelve hours after dosing, prostate tissues were collected for RNA extracti...
ORGANISM(S): Mus musculus 
2014-09-16 | E-GEOD-61416 | biostudies-arrayexpress
ProteomicsQuantitative Proteomics Analysis of Rat Brown Adipose Tissue Based on TMT Labelling
This study employed mouse brown adipose tissue as the sample, utilising TMT labelling combined with LC-MS/MS technology to conduct qualitative identification and relative quantitative analysis of its proteome. By comparing protein expression profiles between different treatment groups (control vs. c...
ORGANISM(S): Rattus norvegicus (Rat) 
2026-08-27 | PXD071045 | Pride
Lc-ms/ms Rat Brown adipose tissue 
ProteomicsQuantitative N-terminome of N-end rule mutant in Arabidopsis
According to the /N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Here, we undertook a quantitative proteomics study of N-end rule mutant prt6, to investigate the impact of this pathway on the etiolated seedli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-10-27 | PXD006450 | Pride
N-terminome Arabidopsis N-end rule mutant etiolated seedlings tmt- labelling tails 
ProteomicsEvaluation of a Dual Isolation Width Acquisition (DIWA) method for isobaric labelling ratio decompression
Stable isotope labelling of peptides using isobaric reagents such as iTRAQ and TMT enables the multiplexed analysis of proteomes with deep quantitative coverage. Isobaric tagging demonstrates high precision but imperfect accuracy due to ratio underestimation caused by co-fragmentation of ions with m...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2019-01-07 | PXD010571 | Pride
Method development Tmt Msms Diwa 
ProteomicsMass spectrometry analysis of estrogen receptor α -interacting proteins.
Quantitative mass spectrometry analysis using tandem mass tags (TMT) labelling of estrogen receptor α (ERα) pull downs. Co-immunoprecipitated proteins using a anti Erα antibody were compared quantitatively with material recovered in a mock to identify ERα-interacting proteins.
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD025018 | Pride
Estrogen receptor Mrna binding Tmt Immunoprecipitation 
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