Sort   by:  
 Page size 
ProteomicsLabeling Escherichia coli to saturation with 2.5 % of 15N ammonium in two conditions (aerobic and anaerobic) for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms after labeling them with stable isotope-labeled substrates. For this dataset Escherichia coli cultures were labeled with 2.5 % of 15N-labeled NH4Cl and grown either anaer...
ORGANISM(S): Escherichia coli 
2022-02-16 | PXD024288 | Pride
Nitrogen 15 Stable isotope probing Stable isotope 15n Protein-sip 1d-lc Metaproteomics Lc-ms/ms 
TranscriptomicsPhylogenetic patterns in the microbial response to resource availability: amino acid incorporation in San Francisco Bay
Aquatic microorganisms are typically identified as either oligotrophic or copiotrophic, representing trophic strategies adapted to low or high nutrient concentrations, respectively. Here, we sought to take steps towards identifying these and additional adaptations to nutrient availability with a qua...
ORGANISM(S): marine metagenome 
2014-03-26 | E-GEOD-56119 | biostudies-arrayexpress
Proteomics15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome
AtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modif...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-12-02 | PXD020700 | Pride
Seedling Acinus Plant Arabidopsis thaliana Atacinus Co-immunoprecipitation 15n stable-isotope-labeling in arabidopsis(silia) Ip-ms Lc-ms/ms 
UnknownLaboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies.
Not available
S-EPMC6704667 | biostudies-literature
Proteomics15N labeling of mouse microbiota
This study investigated the in vivo dynamics of the microbiome using metaproteomics coupled with protein-SIP in mice.
ORGANISM(S): gut metagenome 
2021-08-26 | PXD017451 | Pride
Microbiota Mouse 15n stable isotope labeling Metaproteomics 
ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
MetabolomicsMetabolic differences between primary and brain metastatic breast tumors revealed by in vivo [U-¹³C]-glucose infusion in mice

Metastatic breast tumors must adapt to the metabolic constraints of their target tissues. To understand how tumor metabolism varies across sites, we compared glucose utilization in primary mammary fat pad (MFP) tumors and brain metastases derived from the breast cancer cell line MDA-MB-231. Tumor...

2025-12-30 | MTBLS13152 | MetaboLights
Proteomics15N Limitation of detection in Pseudomonas fluorescens
The limit of 15N detection in proteins of Pseudomonas putida was studied by LC-MS/MS. Cells were grown in the presence of 0.1%15N to 10% 15N.The method of protein-based stable isotope probing (protein-SIP) has previously been shown to allow the modeling of carbon fluxes in microbial communities, dem...
ORGANISM(S): Pseudomonas fluorescens 
2013-04-09 | PXD000127 | Pride
Protein-sip 15n Lc-msms 
ProteomicsQuantitative Tracking of Isotope Flows in Proteomes of Microbial Communities
Stable isotope probing (SIP) has been used to track nu- trient flows in microbial communities, but existing pro- tein-based SIP methods capable of quantifying the degree of label incorporation into peptides and proteins have been demonstrated only by targeting usually less than 100 proteins per samp...
ORGANISM(S): Escherichia coli 
2024-06-14 | PXD041958 | Pride
Proteomics 15n labeling Stable isotope Sip 
ProteomicsA protocol for global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics
Differential proteomics targeting the abundance level and changes of proteins is commonly used in biology to determine changes induced by different physiological states in living organisms. Here, current methods comprise both label-free and label-based proteomics approaches. More specifically, besid...
ORGANISM(S): Bacillus pumilus 
2017-10-02 | PXD005262 | Pride
Peroxide stress adaptation Gel-based proteomics Phosphorylation 14n/15n metabolic labeling Bacillus pumilus Quantification 
Sort   by:  
 Page size