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Proteomics20S proteasome digestion of HeLa cell extracts
The 20S proteasome is responsible for the catalytic activity of all proteasome complexes. Structural constraints mean that only unfolded, extended polypeptide chains may enter the catalytic core of the 20S proteasome. Here we conducted a comprehensive analysis of the 20S CP substrates in-vitro. We r...
ORGANISM(S): Homo sapiens (Human) 
2018-07-30 | PXD010132 | Pride
20s proteasome digestion Heat soluble proteins 
ProteomicsSystematic identification of 20S proteasome targets
For many years, the ubiquitin-26S proteasome degradation pathway was considered the principal route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by an ubiquitin-independent mechanism mediated by the core 20S proteasome itself. The ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-21 | PXD044399 | Pride
Disorder Degradation 20s proteasome 
ProteomicsUbiquitin remenants identification following in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD028912 | Pride
20s Ubiquitin Proteasome 
ProteomicsKRAS G12V digestion by 20S proteasome
KRAS is a well known tumor associated antigen. It’s a GTPase that functions as molecular switch in regulatory pathways responsible for proliferation and survival. It’s frequently mutated in a variety of cancers. One of the possible driver mutations is G12V substitution, which impairs KRAS GTPase act...
ORGANISM(S): Homo sapiens (Human) 
2019-10-24 | PXD015580 | Pride
Proteasome Cancer Kras Digestion Lc-ms/ms 
ProteomicsSignature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia
Careful removal of unwanted proteins is necessary for cell survival. The primary constitutive intracellular protease is the 26S proteasome complex, very often found in equilibrium with its catalytic core particle – the 20S subcomplex. Protein degradation by the former is tightly regulated due to pri...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018722 | Pride
26s 20s Peptides Proteasome Hypoxia 
TranscriptomicsSynergistic effects of TOR and proteasome pathways on the yeast transcriptome and cell growth
The experiments were designed to interrogate the genetic interaction relationship between the TOR signalling pathway and the UPS-proteasome pathway in gene expression and cell growth. Yeast cells (BY4742pdr5delta) were treated with the drug vehicle, MG132 (50uM), rapamycin (200ng/ml) or both drugs. ...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-24 | E-MTAB-1550 | biostudies-arrayexpress
ProteomicsAnalysis of the fragments from hTau degraded by proteasome 20s using mass spectrometry
We found two stable intermediates in the interaction of human Tau and proteasome 20s. The aim of this study is to determine the cleavage sites on hTau during this process.
ORGANISM(S): Escherichia coli 
2020-04-22 | PXD015349 | Pride
Htau Mass spectrometry 
ProteomicsNEDDylation promotes nuclear protein aggregation and protects the Ubiquitin Proteasome System upon proteotoxic stress
SILAC quantitative mass spectrometry analysis on protein aggregates induced upon heat shock. Two separate performed analysis: A) control siRNA cells (light) with siRNA NEDD8 cells (heavy) and B) control cells (light) with MLN4924 treated cells (heavy). In all conditions cells were heat shocked at 43...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-17 | MSV000082623 | MassIVE
ProteomicsMass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells
Analysis of 20S proteasome in various tissue, adipose derived stem cells(ADSC) or HeLa cells treated with interferon-gamma. This results are compared with LC-SRM results obtained on the same samples. Global proteomics on ADSC amplified in 5% or 20% O2.
ORGANISM(S): Homo sapiens (Human) 
2019-02-14 | PXD011894 | Pride
Human Adsc Proteasome Tissue 
ProteomicsDigestion of TSN2 and TSN89 synthetic peptides by proteasomes
Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or ligate non-contiguous peptide fragments thereby generating novel sequences, i.e. spliced peptides via a process called prote...
ORGANISM(S): Homo sapiens (Human) 
2022-12-19 | PXD025995 | Pride
Synthetic peptides Proteasome Digestion Lc-ms/ms 
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