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MetabolomicsComparative non-targeted LC-MS metabolomic profiling of murine bone marrow-derived macrophages cultured in 2D versus 3D

Macrophages are key drivers of inflammatory and fibrotic diseases and their activation is shaped by interactions in their tissue microenvironment. However, dissecting the processes that drive immunopathology has proved challenging as traditional 2D culture methods fail to capture the complex mole...

2026-06-08 | MTBLS12917 | MetaboLights
ProteomicsComparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD009860 | Pride
Mass spectrometry Breast cancer Breast milk 2d-page Proteomics Biomarkers 
TranscriptomicsMadin-Darby Canine Kidney (MDCK) cells grown in 2-dimensional and 3-dimensional type I collagen culture.
In order to gain insight into epithelial morphogenesis and the influence of culture geometry on gene expression patterns, Madin Darby Canine Kidney (MDCK) epithelial cells where grown in 2-dimensional (2D) culture or 3-dimensional (3D) culture . MDCK cells cultured in 2D were plated atop a pre-soli...
ORGANISM(S): Canis lupus familiaris 
2013-06-03 | E-GEOD-28381 | biostudies-arrayexpress
ProteomicsProteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoressi (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk
Breast Cancer (BC) is one of the most common cancers and one of the most common causes for cancer- related mortality. Discovery of protein biomarkers associated with cancer, is considered as important for early diagnosis and prediction of the cancer risk. Protein biomarkers could be investigated by ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-20 | PXD040188 | Pride
Breast cancer  breast milk  biomarkers  protein dysregulation  2d-page  proteomics  mass spec-trometry 
ProteomicsAgriope Proteins from 2D-Gel and LC-ESI
Agriope Proteins from 2D-Gel and LC-ESI-MS approach
ORGANISM(S): Bos taurus (Bovine) 
2021-09-09 | PXD018693 | Pride
Maldi-tof ms  lc-esi-ms 2-d gelelectrophoresis 
ProteomicsIn-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensi...
ORGANISM(S): Dermatophagoides Pteronyssinus Saccharomyces Cerevisiae 
2024-01-26 | PXD045568 | panorama
ProteomicsHuman intracranial tumors LC-MS/MS
We found the reality that each Coomassie blue-stained 2D gel spot contained lots of protein species in analysis of in tissue proteome.
ORGANISM(S): Homo sapiens (Human) 
2017-12-11 | PXD004930 | Pride
Human Lc-ms/ms Intracranial tumors 2d-gel 
ProteomicsArabidopsis plastoglobules 2D-gel separation
Protein complexes separation of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype). PGs were isolated from the plants when they were 4 weeks old under long day photoperiod ( during the morning in dark adapted plants,16 hours of light and 8 darkness photoperiod, LED light of 120 ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-04-13 | PXD019765 | Pride
Pgs Arabidopsis 
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all t...
ORGANISM(S): Histomonas meleagridis 
2018-11-22 | PXD009332 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
ProteomicsCircadian profiling of the Arabidopsis proteome using 2D-DIGE
Clock-generated biological rhythms provide an adaptive advantage to an organism which results in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under constant light. Using large-scale two-dimen...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-06-29 | PXD004276 | Pride
Arabidopsis Circadian clock 2d-dige 
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