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ProteomicsPhosphorylated RNAPII interactome via 4H8 antibody IP
4H8 pull down of chromatin-associated proteins in the Flp-In T-REx HEK293 parental cell line. Phosphorylated RNAPII interactome was measured in three conditions; Untreated, 1mM H2O2 treatment for 15minutes, and 1 hour recovery
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD067969 | Pride
4h8 Interactome Phosphorylated rnapii 
ProteomicsAnti-Pol II pS5 co-immunoprecipitation in PHF3 WT, KO and ΔSPOC HEK293T cells
The analysis of differential interactome of Pol II pS5 in PHF3 WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (4H8) antibody coupled to Protein G beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-25 | PXD039537 | Pride
Rna pol ii ps5 Co-immunoprecipitation Phf3 
GenomicsGenome-wide RNA Pol II-CTD occupancy in wild type and mutant worms
We used RNA Pol II-CTD occupancy assay to identify mRNA isoform switch in mutant animals compared to wild type animals. Examination of RNA Pol II-CTD Ser5P recognized by 4H8 in wild type, sydn-1(0), ssup-72(0) and ssup-72(0);sydn-1(0)
ORGANISM(S): Caenorhabditis elegans 
2015-11-01 | E-GEOD-68203 | biostudies-arrayexpress
ProteomicsPhosphorylated RNAPII interactome via 4H8 antibody IP with OGG1 inhibitor
RNAPII pull down of chromatin associated proteins in the Flp-In T-REx HEK293 parental cell line. Phosphorylated RNAPII interactome was measured in six conditions; Untreated (with and without OGG1i), 1mM H2O2 treatment for 15minutes (with and without OGG1i) and 2 hours recovery following media washo...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD070086 | Pride
Ogg1i 4h8 Interactome Phosphorylated rnapii 
GenomicsMediator promotes CENP-A incorporation at fission yeast centromeres [ChIP-seq]
At Schizosaccharomyces pombe centromeres, heterochromatin formation is required for de novo incorporation of the histone H3 variant CENP-A/Cnp1, which in turn directs kinetochore assembly and ultimately chromosome segregation during mitosis. Noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (...
ORGANISM(S): Schizosaccharomyces pombe 
2012-11-06 | E-GEOD-35524 | biostudies-arrayexpress
GenomicsChIP-Seq of RNA Pol II with antibodies S2, 4H8 and 8WG16 during C. elegans L1 arrest and recovery
When C. elegans larvae hatch in the absence of food they persist in a stress resistant, developmentally arrested state (L1 arrest) for weeks or until food becomes available. We characterized growth, mRNA expression, and RNA Polymerase II activity genome-wide during L1 arrest and recovery. RNA Pol ...
ORGANISM(S): Caenorhabditis elegans 
2009-02-27 | E-GEOD-13973 | biostudies-arrayexpress
GenomicsDesai PolII-4H8
modENCODE_submission_174 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expressio...
ORGANISM(S): Caenorhabditis elegans 
2010-07-07 | GSE22669 | GEO
UnknownFluorescence correlation spectroscopy as a sensitive and useful tool for revealing potential overlaps between the epitopes of monoclonal antibodies on viral particles.
Not available
S-EPMC5058619 | biostudies-literature
GenomicsChIP-Seq of RNA Pol II with antibodies S2, 4H8 and 8WG16 during C. elegans L1 arrest and recovery
When C. elegans larvae hatch in the absence of food they persist in a stress resistant, developmentally arrested state (L1 arrest) for weeks or until food becomes available. We characterized growth, mRNA expression, and RNA Polymerase II activity genome-wide during L1 arrest and recovery. RNA Pol II...
ORGANISM(S): Caenorhabditis elegans 
2009-02-27 | GSE13973 | GEO
GenomicsRNA polymerase II collision interrupts convergent transcription (ChIP-seq)
Anti-sense non-coding transcripts, genes-within-genes, and convergent gene pairs are prevalent among eukaryotes. The existence of such transcription units raises the question of what happens when RNA polymerase II (RNAPII) molecules collide head-to-head. Here we use a combination of biochemical and ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-11-30 | E-GEOD-38382 | biostudies-arrayexpress
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