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ProteomicsDual-Probe Activity-Based Protein Profiling Reveals Site-Specific Binding Dynamics of EGFR-Directed Drugs
Comparative, dose-dependent analysis of interactions between small molecule drugs and their targets, as well as off-targets, in complex proteomes is crucial for selecting optimal drug candidates. The affinity of small molecules for targeted proteins is largely dictated by interactions between amino ...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD045864 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  concentration-dependent analysis  dual-probe comparison. 
ProteomicsValidation and application of a novel KLK6 activity based probe (IMP2352)
Pancreatic cancer has the lowest survival rate of all common cancers due to late diagnosis and limited treatment options. Serine hydrolases are known to mediate cancer progression and metastasis through initiation of signaling cascades and cleavage of extracellular matrix proteins, and the kallikrei...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD035111 | Pride
Abpp Klk Activity-based probe Kallikrein-related peptidase N-terminomics 
ProteomicsElectrophile scanning by chemical prote-omics reveals a potent pan-active DUB probe for investigation of deubiquitinase activity in live cells
Deubiquitinases (DUBs) are proteases that hydrolyze isopeptide bonds linking ubiquitin to protein substrates, which can lead to reduced substrate degradation through the ubiquitin proteasome system. Deregulation of DUB activity has been im-plicated in many disease states, including cancer, neurodege...
ORGANISM(S): Homo sapiens (Human) 
2024-01-03 | PXD035417 | Pride
Ubiquitin-activity base probe Deubiquitinase Chemical probe Activity-based probe Electrophilic war-head Ubiquitin Dub Enzyme targeting Myc-deregulated cancer 
ProteomicsActivity-based proteomic profiling (ABPP) employing a serine hydrolase-directed probe to identify changes in activity profiles of lipid hydrolase in lung tumors
Lung tumors, as well as normal tumor-adjacent (NTA) tissue of non-small cell lung cancer (NSCLC) patients, were collected and subjected to labeling with a serine hydrolase-directed activity-based probe (ABP) immediately after excision. Labeling with the serine hydrolase-directed ABP allows for the d...
ORGANISM(S): Homo sapiens (Human) 
2023-11-27 | PXD046934 | Pride
Abpp Lipid hydrolysis Serine hydrolase Nsclc Lipid hydrolase Lung tumor Activity-based proteomic profiling Lipase 
ProteomicsZNFX1 activity-based probe crosslinking mass spectrometry
The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture...
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD073419 | Pride
ProteomicsSITE-SPECIFIC ACTIVITY-BASED PROTEIN PROFILING USING PHOSPHONATE HANDLES
Most drug molecules target proteins. Identification of the exact drug binding sites on these proteins is essential to understand and predict how drugs affect protein structure and function. To address this challenge, we developed a strategy that uses IMAC-enrichable phosphonate affinity tags, for ef...
ORGANISM(S): Homo sapiens (Human) 
2023-01-10 | PXD036569 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  phosphonate affinity handles  fe(iii)-immobilized metal affinity chromatography. 
ProteomicsDevelopment of Substrate-Directed Activity-Based Probes via Disulfide-Trapping for Site-Specific Interrogation of Nucleosomal Deubiquitination
Activity-based ubiquitin probes (Ub-ABPs) have been instrumental in interrogating deubiquitinases (DUBs), yet conventional Ub-ABPs lack the substrate-specific and site-specific contextual cues required to investigate nucleosomal DUBs, whose activity is tightly regulated by the chromatin environments...
ORGANISM(S): Homo Sapiens 
2025-12-30 | PXD072417 |
ProteomicsDiscovery of highly Potent and Selective Activity-Based Probes for the Deubiquitylating Enzyme USP30
Ubiquitin-specific protease 30 (USP30) is a deubiquitylating enzyme (DUB) localized in the mitochondrial membrane, which is related to PINK1/Parkin-mediated mitophagy, pexophagy, BAX/BAK-dependent apoptosis, and IKKβ-USP30-ACLY-regulated lipogenesis/tumorigenesis. Mission therapeutics pointed their ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD044792 | Pride
Usp30  activity-based probes  chemical probes  desi  deubiquitinase. 
ProteomicsThe development of activity-based mannanase probes
A Biotin-Man-manno-cyclophellitol activity-based probe (ABP) was used to pull-down putative mannanases from the secretome of the parental strain of A. niger MA234.1 grown for at least 72h after transfer on konjac flour (kF). AnManA was successfully enriched in the secretome. Total protein identifi...
ORGANISM(S): Fungi 
2026-07-17 | PXD076800 | Pride
Cyclophellitol Aspergillus niger Endo-mannanases Activity-based probes 
ProteomicsFunctional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Dysregulated proteolysis plays a pivotal role in the pathophysiology of inflammatory bowel disease. Nonetheless, the identity of overactive proteases released by human colonic mucosa remains unknown. Herein, we employed a serine protease-targeted activity-based probe (ABP) coupled with mass spectral...
ORGANISM(S): Homo sapiens (Human) 
2018-05-18 | PXD009450 | Pride
Inflammation Functional proteomics Activity-based probe Serine protease Inflammatory bowel disease 
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