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ProteomicsAffinity capture of S. cerevisiae protein after glutaraldehyde stabilization
It remains extraordinarily challenging to elucidate endogenous protein-protein interactions and proximities within the cellular milieu. The dynamic nature and the large range of affinities of these interactions augment the difficulty of this undertaking. Among the most useful tools for extracting su...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-19 | PXD001262 | Pride
Budding yeast Glutaraldehyde Affinity capture 
UnknownMultiplexed protein profiling by sequential affinity capture.
Not available
S-EPMC5071697 | biostudies-literature
ProteomicsAffinity capture enrichment versus Affinity depletion: A comparison of strategies for increasing coverage of low abundant human plasma proteins
present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from plasma samples thereb...
ORGANISM(S): Homo sapiens (Human) 
2020-08-27 | PXD020727 | Pride
Affinity enrichment Depletion Human plasma 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until n...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-10-18 | PXD040308 | Pride
Proteomics Capture peptide Capture-ms Centrosomes 
Proteomics14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.
We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to examine the signaling pathways in which 14-3-3 proteins participate and to identify new targets of 14-3-3 signaling during dsRNA-stimulation.
ORGANISM(S): Homo sapiens (Human) 
2014-08-05 | PXD000677 | Pride
Human Itraq Hacat keratinocytes Lc-ms/ms 
TranscriptomicsSelective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo
As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting and folding factors. Here we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Esch...
ORGANISM(S): Escherichia coli 
2011-12-09 | E-GEOD-33671 | biostudies-arrayexpress
ProteomicsImmunoisolation of the trypanosome decapping enzyme ALPH1 delivers a potential subcomplex
Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2...
ORGANISM(S): Trypanosoma brucei 
2023-06-14 | PXD042322 | Pride
Xrna Mrna decay Apah-like phosphatase Alph1 Mrna decapping Trypanosoma Exoribonuclease 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until n...
ORGANISM(S): Homo sapiens (Human) 
2023-10-18 | PXD040309 | Pride
Proteomics Centrosomes Capture ms 
ProteomicsMulti-condition affinity capture/MS analysis of LAP-tagged human NCBP1, NCBP2, and NCBP3
A 5′, 7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and thus a textbook aspect of co-transcriptional pre-mRNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Recently, ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-09 | PXD016038 | Pride
Mrnp Cap binding complex Cbc Interactome screen 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are the major microtubule-organising centers in animals and play fundamental roles in many cellular processes. Understanding how their composition varies across diverse cell types and how it is altered in disease aremajor unresolved questions, yet currently available centrosome isolation...
ORGANISM(S): Homo sapiens (Human) 
2023-09-18 | PXD043906 | Pride
Appendages Centriole Proteome Centrosome 
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