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ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate several functions at once and likely impact the structural integrity of the large mRNP assemblies, these...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD035153 | Pride
Rna-binding sites Npl3 Sr-like protein Mrnp assembly Structure Xl-ms Rna-binding proteins Nmr Mrna Gene expression 
GenomicsPerturbation of mRNP biogenesis reveals a dynamic landscape of the Rrp6-dependent surveillance machinery trafficking along the yeast genome (ChIP-seq dataset)
In order to dissect the nuclear RNA quality control (QC) mechanisms, we used a powerful assay based on global perturbation of mRNP biogenesis in yeast Saccharomyces cerevisiae by the bacterial Rho helicase. We have monitored the global RNA levels upon Rho activation and compare it to basal RNA level...
ORGANISM(S): Saccharomyces cerevisiae 
2019-12-04 | E-MTAB-7539 | biostudies-arrayexpress
TranscriptomicsPerturbation of mRNP biogenesis reveals a dynamic landscape of the Rrp6-dependent surveillance machinery trafficking along the yeast genome (RNA-seq dataset)
In order to dissect the nuclear RNA quality control (QC) mechanisms, we used a powerful assay based on global perturbation of mRNP biogenesis in yeast Saccharomyces cerevisiae by the bacterial Rho helicase. We have monitored the global RNA levels upon Rho activation and compare it to basal RNA level...
ORGANISM(S): Saccharomyces cerevisiae 
2019-12-04 | E-MTAB-7538 | biostudies-arrayexpress
Transcriptomics3' UTR length and mRNP composition determine endocleavage efficiencies at termination codons
We aimed to study the cleavage sites of nonsense-mediated mRNA decay (NMD) substrates. Therefore, we depleted the major exoribonuclease XRN1 in human cell culture, which degrades the 3' fragments generated by SMG6-mediated endonucleolytic cleavage. Different reporter mRNAs or endogenous NMD targets ...
ORGANISM(S): Homo sapiens 
2014-11-11 | E-MTAB-2907 | biostudies-arrayexpress
TranscriptomicsTranscriptome maps of mRNP biogenesis factors define pre-mRNA recognition
Biogenesis of eukaryotic messenger ribonucleoprotein complexes (mRNPs) involves the synthesis, splicing, and 3M-bM-^@M-^Y-processing of pre-mRNA, and the assembly of mature mRNPs for nuclear export. We mapped 23 mRNP biogenesis factors onto the newly synthesized yeast transcriptome, providing ~10^5-...
ORGANISM(S): Saccharomyces cerevisiae 
2014-07-23 | E-GEOD-59676 | biostudies-arrayexpress
ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like pr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD034656 | Pride
Affinity purification Rna-processing Lfq Npl3 Spliceosome 
GenomicsTranscriptome-wide view of endosomal mRNP transport [iCLIP ]
Transcriptome-wide view of endosomal mRNP transport [iCLIP ]
PRJNA431398 | ENA
UnknownStatistical modeling of mRNP transport in dendrites: A comparative analysis of β-actin and Arc mRNP dynamics.
Not available
S-EPMC10946522 | biostudies-literature
GenomicsTranscriptome-wide view of endosomal mRNP transport [RNA-Seq]
Transcriptome-wide view of endosomal mRNP transport [RNA-Seq]
PRJNA431397 | ENA
UnknownNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.
Not available
S-EPMC9881175 | biostudies-literature
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