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TranscriptomicsA single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast
Mammalian SR proteins are a family of reversibly phosphorylated RNA binding proteins primarily studied for their roles in alternative splicing. While budding yeast lack alternative splicing, they do have three SR-like proteins: Npl3, Gbp2, and Hrb1. However, these have been primarily studied for the...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-30 | E-GEOD-11491 | biostudies-arrayexpress
TranscriptomicsExpression data from Saccharomyces cerevisae strains deleted for the RNA-binding hnRNP Npl3
Transcription is a major obstacle for replication fork progression and a cause of genome instability. Such instability increases in mutants with a suboptimal assembly of the nascent messenger ribonucleo-protein particle (mRNP), as THO/TREX and some heterogeneous nuclear ribonucleoproteins (hnRNPs) m...
ORGANISM(S): Saccharomyces cerevisiae 
2013-11-15 | E-GEOD-50186 | biostudies-arrayexpress
ProteomicsLC-MS/MS analysis of p-Npl3 experiment
The purpose of this experiment is to analyze the variable modification of phospho (STY) in recombinant p-Npl3
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-04-20 | PXD024494 | Pride
Lc-msms Npl3 Yeast 
ProteomicsNpl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like pr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD034656 | Pride
Affinity purification Rna-processing Lfq Npl3 Spliceosome 
GenomicsMetamycoplasma hyosynoviae strain:NPL3
Mycoplasma hyosynoviae NPL3 Genome sequencing
PRJNA238375 | ENA
TranscriptomicsLoss of the yeast SR protein Npl3 alters gene expression due to transcription readthrough
Yeast Npl3 is a highly abundant RNA binding protein, related to metazoan SR proteins, with reported functions including transcription elongation, splicing and RNA 3’ end processing. To identify direct targets and functions for Npl3, we used UV crosslinking and analysis of cDNA (CRAC) to map precise ...
ORGANISM(S): Saccharomyces cerevisiae S288c 
2015-12-02 | E-MTAB-3642 | biostudies-arrayexpress
GenomicsrRNA processing is surveilled by the mRNA guard protein Npl3
rRNA processing is surveilled by the mRNA guard protein Npl3
PRJNA1114745 | ENA
ProteomicsNpl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence
Telomere shortening rates must be regulated to prevent premature replicative senescence. TERRA R-loops become stabilized at critically short telomeres to promote their elongation through homology-directed repair (HDR), thereby counteracting senescence onset. Using a non-bias proteomic approach to id...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-01-31 | PXD016772 | Pride
Lc-ms/ms  telomere  pulldown 
TranscriptomicsThe structure and target identification of the tandem RRM of yeast Npl3 bound to RNA reveal a cooperative sequence-specific recognition and an RNA chaperone role in splicing
The conserved SR-like protein Npl3 promotes the splicing of diverse pre-mRNAs. However, the RNA sequence(s) recognized by the RNA Recognition Motifs (RRM1 & RRM2) of Npl3 during the splicing reaction remain elusive. Here, we developed a split-iCRAC approach in vivo to determine the consensus sequenc...
ORGANISM(S): Saccharomyces cerevisiae 
2022-12-01 | E-MTAB-11736 | biostudies-arrayexpress
GenomicsThe SR-like protein Npl3 transfers mRNP components from the transcription site onto the mRNA
The SR-like protein Npl3 transfers mRNP components from the transcription site onto the mRNA
PRJNA674049 | ENA
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