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ProteomicsGenome-wide CRISPR screens and quantitative proteomics reveal remodelling of the aryl hydrocarbon receptor-controlled proteome through PARP7 activity
PARP7 is an enzyme that uses donor substrate NAD+ to attach a single ADP-ribose moiety onto proteins related to immunity, transcription, and cell growth and motility. Despite the importance of PARP7 in these processes, PARP7 signalling networks remain under-researched. Here, we used genome-wide CRIS...
ORGANISM(S): Homo sapiens (Human) 
2025-05-11 | PXD062520 | Pride
Screen Proteomics Ahr Mass spectrometry Parp Crispr Adp-ribosylation Aryl hydrocarbon receptor 
ProteomicsSerine ADP-ribosylation depends on HPF1
We report that serine ADPr is strictly dependent on HPF1 (histone PARylation factor 1. Quantitative proteomics revealed that serine ADPr does not occur in cells lacking HPF1. We identified three endogenous serine ADPr sites are located on the PARP-1 automodification domain. Further identification of...
ORGANISM(S): Homo sapiens (Human) 
2017-02-10 | PXD005627 | Pride
Adp-ribosylation  hpf1  parp-1  parp-2  histones  proteomics 
ProteomicsHistone ADP Ribosylation - Serine is a new target residue for endogenous ADP-ribosylation on histones
ADPr sites on histones obtained from cells were directly identified. We have identified 12 unique ADPr sites in human osteosarcoma cells and report serine ADPr as a new type of histone mark that responds to DNA damage.
ORGANISM(S): Homo sapiens (Human) Gallus gallus (Chicken) 
2016-12-22 | PXD005462 | Pride
Adpr Histones Serine Adp ribosylation 
ProteomicsIdentification of mono-ADPr readers by multilevel chromatin proteomics
We performed multilevel proteomics to identify mono-ADPr readers. Pulldowns using ADPribosylated peptides and nucleosomes, analysis of the chromatin proteome using targeted modulations of the ADPr system (H2O2 treatment, ARH3 KO, HPF1 KO). We reveal multiple mono-ADPr readers, including RNF114, a ...
ORGANISM(S): Homo sapiens (Human) 
2023-04-28 | PXD037026 | Pride
Adpribosylation Histone marks Human Ptm Mono-adpr Chromatin Lc-ms/ms 
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