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ProteomicsDehydroglutathione to induce glutathione modification in protein
Protein S-glutathionylation is one of the major cysteine oxidations regulating redox signaling and oxidative stress. In this study, we developed a glutathione-derived chemical tool, namely dehydroglutathione, that can introduce a non-reducible glutathionylation mimic to protein, which can be utilize...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061319 | Pride
Glutathionylation Fabp5 Dehydroglutathione 
ProteomicsClickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation
Identification of cysteines with high oxidation susceptibility is important for understanding redox-mediated biological processes associated with health and disease. We developed a chemical proteomic strategy that helps find cysteines with high susceptibility to S-glutathionylation. Our strategy is ...
ORGANISM(S): Mus musculus (Mouse) 
2024-03-07 | PXD034976 | Pride
Lc-ms/ms for glutathionylation in mouse c2c12 
ProteomicsSite-specific Identification of Glutathionylation
Cardiomyocytes proteins were investigated for their glutathionylation in response to hydrogen peroxide generated from glucose oxidase and using a clickable glutathione derivative. After exposure, or not, to hydrogen peroxide the glutathionylated proteins were enriched and LC-MS/MS analyses was perfo...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-06 | PXD012171 | Pride
Ptm Glutathionylation Affinity purification 
ProteomicsSite-specific Identification and Quantification of Protein Glutathionylation under Ischemic Stress
Ischemia or ischemic reperfusion is well-known for its contribution to heart diseases. During ischemia and reperfusion, a flux of nutrients and oxygen to cardiomyocytes is altered, which causes a burst of ROS from mitochondria and other enzymes. Elevated levels of ROS can cause oxidative modificatio...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-13 | PXD024766 | Pride
Gutathionylation Cardiomyocytes Click chemistry 
ProteomicsIsotopic-labelled Clickable Glutathione to Quantify Protein S-Glutathionylation
Investigated protein glutathionylation in HL-1 cardiomyocyte cells in response to hydrogen peroxide using a clickable glutathione approach. After exposure, or not, to hydrogen peroxide the glutathionylated proteins with heavy or light azido glutathione were enriched and subjected to LC-MS/MS analysi...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD014937 | Pride
Clickable glutathione Glutathionylation Cardiomyocyte 
ProteomicsIn-vitro glutathionylation of SMYD2
Glutathionylation is an important post-translational modification and developing tools to identify which cysteine residues on proteins are glutathionylated in response to different physiological conditions is necessary. This project utilizes an approach consisting of azido-glutathione, a single puri...
ORGANISM(S): Homo sapiens (Human) 
2019-04-03 | PXD011123 | Pride
Human Cysteine Glutathionylation Purified protein Click chemistry 
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