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TranscriptomicsComparison of Differences in Mouse Mammary gland gene expression data at POSTNATAL DAY 70 in Akt1wildtype versus Akt1-deficient mice
Akt1, a serine-threonine protein kinase member of the PKB/Akt gene family, plays a critical role in the regulation of several cellular processes including cell proliferation and apoptosis. In this study, we utilized Akt1+/+ and Akt1¬-/- C57/Bl6 female mice to demonstrate that Akt1 is required for n...
ORGANISM(S): Mus musculus 
2011-07-14 | E-GEOD-30671 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mammary glands from wild type and Akt1 knock out lactating mice at day 9 after parturition
A comparison of gene expression in the mammary gland of lactating mice at day 9 after parturition between Akt -/- and wildtype individuals.
ORGANISM(S): Mus musculus 
2006-12-31 | E-MEXP-891 | biostudies-arrayexpress
ProteomicsATP-competitive and allosteric inhibitors induce differential conformational changes at the autoinhibitory interface of Akt
We use HDX-MS to interrogate the AKT1 DrLink conformational changes upon binding AKT1 active site inhibitors A-443654, Capivasertib, and Uprosertib, Akt1 allosteric inhibitor MK-2206, and ADP.
ORGANISM(S): Homo sapiens (Human) 
2024-08-23 | PXD039028 | Pride
Akt1 drlink Atp competitive inhibitors Allosteric inhibitors Akt1 Protein kinase b Hdx-ms 
ProteomicsDelineating changing partners of Akt1 protein as a function of its active state in HEK-293 Cells
Akt1 is a multi-functional protein, implicated and activated in multiple human solid tumors. Functional diversity of Akt1 is a result of its interactions with other proteins; which changes with changing context. This study was designed to capture the dynamics of Akt1 Interactome as a function of its...
ORGANISM(S): Homo sapiens (Human) 
2017-03-02 | PXD005976 | Pride
Apms Silac Akt1 Interactome 
TranscriptomicsTranscription profiling of hearts from DTG-positive mice before and after the induction of the transgene Akt1 at different time points to investigate molecular mechanisms involved in the development of cardiac hypertrophy and heart failure
To investigate molecular mechanisms involved in the development of cardiac hypertrophy and heart failure, a tetracycline-regulated transgenic system to conditionally switch a constitutively-active form of the Akt1 protein kinase on or off in the adult heart was developed. Short-term activation (2 we...
ORGANISM(S): Mus musculus 
2007-12-01 | E-GEOD-3383 | biostudies-arrayexpress
ProteomicsDefining the Akt1 interactome and delineating alterations in its composition as a function of cell cycle progression.
Akt1 expressing Hek 293 cells were SILAC labeled to capture dynamic changes in Akt1 interactome as the cell cycle progresses from G0 to G1S and then G2 phase. This will help in understanding how Akt1 extends its regulatory effect upon cell cycle progression.
ORGANISM(S): Homo sapiens (Human) 
2017-01-16 | PXD005557 | Pride
Ap-ms Silac Akt1 Cell cycle Interactome 
ProteomicsAkt1 depletion in murine ovarian granulosa cells reveals known and novel molecular and cellular processes dependent on this kinase
The PI3K/AKT signaling pathway is known to regulate a broad range of cellular processes and it is often altered in several types of cancers. Recently, somatic AKT1 mutations leading to a strong activation of this kinase have been reported in juvenile granulosa cell tumors. However, the molecular rol...
ORGANISM(S): Mus musculus (Mouse) 
2019-04-30 | PXD012548 | Pride
Proteomics Knock-down Akt1 Mouse Transcriptomics Granulosa cells 
TranscriptomicsTranscription profiling of mouse skeletal muscle-specific, conditional transgenic mouse expressing a constitutively-active form of Akt1
In contrast to the well-established role of oxidative muscle fibers in regulating fatty acid oxidation and whole body metabolism, little is known that about the function of fast/glycolytic muscle fibers in these processes. Here, we generated a skeletal muscle-specific, conditional transgenic mouse e...
ORGANISM(S): Mus musculus 
2008-06-16 | E-GEOD-9484 | biostudies-arrayexpress
ProteomicsStructure of autoinhibited Akt1 reveals mechanism of PIP 3 -mediated activation
To directly observe the conformational changes in Akt1 3P elicited by PIP 3 binding, we performed HDX-MS analysis of Akt1 3P in the presence of plasma membrane-mimicking liposomes (20% cholesterol, 30% phosphatidylcholine, 15% phosphatidylserine, 35% phosphatidylethanolamine) that contained either 0...
ORGANISM(S): Homo sapiens (Human) 
2021-09-01 | PXD022061 | Pride
Pip3 Akt Lipids Hdx-ms 
TranscriptomicsmiRNA arrays for LPS-activated Akt1+/+ and Akt1-/- macrophages
MicroRNAs regulated by lipopolysaccharide (LPS) target genes that contribute to the inflammatory phenotype. Here we showed that the protein kinase Akt1, which is activated by LPS, positively regulated miRNAs let-7e, miR-181c but negatively regulated miR-155 and miR-125b. In silico analyses and trans...
ORGANISM(S): Mus musculus 
2009-08-21 | E-GEOD-17421 | biostudies-arrayexpress
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