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TranscriptomicsTranscription profiling of human liver to evaluation of RNA binding specificity of aminoglycosides with DNA microarrays
We have developed methods for using DNA array technology to probe the entire transcriptome to determine RNA-binding specificity of ligands. Two methods were investigated. In the first, the RNA-binding aminoglycoside antibiotic tobramycin was covalently linked to magnetic beads. The beads were bou...
ORGANISM(S): Homo sapiens 
2008-06-13 | E-GEOD-5111 | biostudies-arrayexpress
ProteomicsTranslation Error Clusters Induced by Aminoglycoside Antibiotics
Aminoglycoside antibiotics target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal aminoglycosides induce not only single errors, but also clusters ...
ORGANISM(S): Escherichia coli 
2021-04-06 | PXD022098 | Pride
Aminoglycosides 
ProteomicsTranslation Error Clusters Induced by Aminoglycoside Antibiotics
Aminoglycoside antibiotics (AGAs) target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal AGAs induce not only single translation errors, but also c...
ORGANISM(S): Escherichia Coli 
PXD019328 | panorama
TranscriptomicsTranscriptional response to aminoglycoside antibiotics in cochlear hair cells at 3 hour
We purified Atoh1-GFP positive hair cells from organotypic cultures of P1 cochlea 3 hours after 0.5mM gentamicin treatment and performed RNA sequencing to profile the early transcriptional response of hair cells to aminoglycoside antibiotics. Levels of mRNA in gentamicin-treated hair cells (three re...
ORGANISM(S): Mus musculus 
2015-04-30 | E-GEOD-66775 | biostudies-arrayexpress
ProteomicsTranslation Error Clusters Induced by Aminoglycoside Antibiotics
Aminoglycoside antibiotics (AGAs) target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal AGAs induce not only single translation errors, but also c...
ORGANISM(S): Escherichia coli 
2021-04-06 | PXD019188 | Pride
Antibiotics Miscoding Ribosome Streptomycin Aminoglycoside E. coli Misreading 
TranscriptomicsTranscription profiling of Pseudomonas aeruginosa representational genomic libraries to identify aminoglycoside resistance genes
For this study, we created three highly representational different sized genomic libraries of P. aeruginosa PAO1 within the vector pBTB-1. Vector pBTB-1 is a low copy number broad host range plasmid containing a ß-lactamase resistance marker, a pBAD promoter upstream of the cloning site, as well as...
ORGANISM(S): Pseudomonas aeruginosa 
2009-12-19 | E-MEXP-2396 | biostudies-arrayexpress
GenomicsCo-occurrence of aminoglycoside and beta-lactam resistance in E.coli
Co-occurrence of aminoglycoside and beta-lactam resistance in E.coli
PRJEB36139 | ENA
GenomicsTraDIS in aminoglycoside-resistant E. coli MG1655
Genome-wide analysis of fitness-genes in aminoglycoside-resistant Escherichia coli during antibiotic treatment
PRJEB70315 | ENA
ProteomicsMETABOLIC DISRUPTION IMPAIRS RIBOSOMAL PROTEIN LEVELS, RESULTING IN ENHANCED AMINOGLYCOSIDE TOLERANCE
Aminoglycoside antibiotics display broad-spectrum activity against Gram-negative and Gram-positive bacteria by targeting their ribosomes. Herein, we have demonstrated that energy metabolism plays a crucial role in aminoglycoside tolerance, as knockout strains associated with the tricarboxylic acid c...
ORGANISM(S): Escherichia coli 
2024-06-21 | PXD053082 | Pride
Ribosomal proteins Lc-ms Tca cycle E. coli 
ProteomicsRapid and accurate detection of aminoglycoside modifying enzymes and 16S ribosomal RNA methyltransferases by targeted LC-MS/MS
New and rapid diagnostic methods are needed for the detection of antimicrobial resistance to aid in the curbing of drug-resistant infections. Targeted LC-MS/MS is a method that could serve this purpose, as it can detect specific peptides of resistance mechanisms with high accuracy. In the current st...
ORGANISM(S): Escherichia coli Klebsiella pneumoniae BIDMC 40 
2021-04-30 | PXD024339 | Pride
E. coli Aminoglycosides Parallel reaction monitoring Aminoglycoside modifying enzymes Liquid chromatography mass spectrometry K. pneumoniae 16s ribosomal rna methyltransferases Antimicrobial resistance 
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