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ProteomicsNdd1 phosphorylation pattern upon DNA damage
The transcriptional activator NDD1 was purified from wt and rad53 or mec1 mutant treated with hydroxyurea or methyl-methanesulfonate via HTB-tag , digested directly from the streptavidin beads, and then subjected to LC-MSMS analysis. Beads were alternatively digested with trypsin and chymotrypsin f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2013-12-19 | PXD000405 | Pride
Hydroxyurea Transcription factor Ndd1 Mms Dna damage 
ProteomicsMass spectrometry analysis of in vitro Tau ubiquitination by the UBA1/Hsp70/CHIP machinery
We aimed at characterizing which lysines on Tau were ubiquitinated by the UBA1/Hsp70/CHIP machinery. We also determined the ubiquitin linkages of the assembled Tau chains.
ORGANISM(S): Homo sapiens (Human) 
2024-06-18 | PXD034527 | Pride
Ubiquitination Protein degradation Alzheimer’s disease Tau 
ProteomicsTargeted mass spectrometry of Tau preparations purified from Alzheimer’s Disease brains plus either the deubiquitylase USP21 or its activity-dead mutant C221A.
To characterise the abundance of ubiquitylated Tau peptides in the presence of active or inactive variants (wt 196-565 vs. C221A) of the broad spectrum deubiquitylase USP21. Tau peptides are obtained from Tau fibrils purified from post mortem human brains diagnosed with Alzheimer’s. Their detection ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-18 | PXD046645 | Pride
Alzheimer’s disease (de)ubiquitination/ubiquitylation Neurodegeneration 
ProteomicsCoIP of Nrl1 from normal and irradiated fission yeast cells
Nrl1-associated proteins were isolated from wild-type cells untreated or exposed to 100Gy of ionising radiation, and then subjected to LC-MSMS analysis. Identifications were carried out using trypsin as protease.
ORGANISM(S): Schizosaccharomyces pombe 
2016-01-04 | PXD001583 | Pride
Fission yeast Dna repair Splicesosome 
ProteomicsCharacterization of the C-terminal post-translational modification pattern of the catalytic C subunit of protein phosphatase 2A by mass spectrometry.
Protein phosphatase 2A (PP2A) is an important regulator of cellular signal transduction pathways. The carboxy-terminal tail of the PP2A catalytic C subunit is thought to be post-translationally modified at three critical sites. Carboxy-terminal methyl-esterification at Leucine 309 is essential for t...
ORGANISM(S): Mus musculus (Mouse) 
2020-04-28 | PXD014879 | Pride
Tyrosine phosphorylation C-terminal protein methylation Protein phosphatase 2a Ptyr307- specific antibodies 
ProteomicsThe oxidoreductase PYROXD1 links human myopathies with pre-tRNA splicing and the unfolded protein response.
PYROXD1 is a largely unstudied essential flavoprotein, whose variants were recently reported to cause myopathies in humans. Here, we report the first biochemical and structural characterization of PYROXD1 and uncover its function as a protector of the tRNA ligase complex from oxidative inactivation....
ORGANISM(S): Homo sapiens (Human) 
2021-06-24 | PXD018831 | Pride
Human Flavoprotein Immunoprecipitation Lc-ms/ms 
ProteomicsPotential effect of L1TD1 on proteomics in HAP1 cells
L1TD1 is a domesticated transposon protein which a RNA-binding capacity. In this project, we aim to investigate the mechanisms through which L1TD1 acts on its RNA targets. First, we first aim to identify the de novo RNA targets of L1TD1 and then is to analyze how proteomics and transcriptomics chang...
ORGANISM(S): Homo sapiens (Human) 
2025-02-11 | PXD047402 | Pride
Hap1 cells Rna-binding protein Stability 
ProteomicsMapping of phosphorylation sites on Sgk3 by LC-MS/MS
The phosphorylation profile of Sgk3 protein expressed either in Sf9 insect cells or HEK 293T human cells was assessed by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026281 | Pride
Activation loop Phosphorylation Sgk3 Turn motif Hydrophobic motif 
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