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Non-targeted metabolomics was performed to investigate the metabolic differences between fertile and sterile wheat anthers, and to identify anther-derived metabolites that promote Fusarium head blight infection. Wheat anthers (fertile, sterile, and fertile anthers co-cultured with Fusarium gramin...

2026-06-23 | MTBLS14831 | MetaboLights
Fusarium head blight is a devastating wheat disease that initiates at flowering, with anthers as the preferred infection site. However, the anther-derived molecules driving this susceptibility remain elusive. Here, we identify anther-enriched phosphatidylcholine, particularly PC(18:2/18:2) and PC(16...
2026-06-23 | MTBLS14814 | MetaboLights
We describe here a new micro-proteomics workflow for the analysis of individual barley anthers. The workflow detects more than 4000 proteins from small amounts of material representing single or paired anther samples, covering a dynamic range of protein expression levels across five orders of magnit...
ORGANISM(S): Hordeum vulgare (Barley) 
2019-05-04 | PXD010887 | Pride
In the current project, we aimed to discover how the barley anther proteome changes, both quantitatively and qualitatively, during meiotic prophase I when the important process of homologous recombination is taking place. Taking advantage of a recently published barley anther transcriptomic dataset ...
ORGANISM(S): Hordeum vulgare (Barley) 
2022-04-04 | PXD026200 | Pride
The study was aimed to investigate by differential proteomics heat stress response mechanisms in tomato anthers collected from flowers of Solanum lycopersicum cv Saladette (SAL), already annotated as a thermo-tolerant tomato genotype and flowers from Solanum lycopersicum cv M82, reported as a thermo...
ORGANISM(S): Solanum lycopersicum 
2018-07-25 | PXD010156 | Pride
Agilent oligonucleotide arrays were used to profile gene expression in dissected maize anthers of 3 types of male-sterile plants and their fertile siblings at four stages of development: after anther initiation, at the rapid mitotic proliferation stage, pre-meiosis, and meiotic prophase I. The male...
ORGANISM(S): Zea mays 
Protein lysine acetylation (KAC) is a dynamic and reversible post-translational modification, playing important biological roles in many organisms.Here, we reported results from a proteomic investigation to detect KAC status of the developing rice anthers near the time of meiosis (RAM), providing st...
ORGANISM(S): Oryza sativa (Rice) 
2019-03-06 | PXD007986 | Pride
Col-0 anthers, control and iron-deficient
Cotton (Gossypium hirsutum L.) is an important economic crop, used mainly for the production of textile fiber. Using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant CCRI9106 was isolated from the wild-type upland cotton cultivar CCRI040029. To use CCRI9106 in ...
ORGANISM(S): Gossypium hirsutum 
2015-06-11 | PXD002209 | Pride
OJAP_WD199 was annotated as partial WD40 genes, whereas showed strong pollen-specific expression. We generated CRISPR-Cas9-induced loss-of-function mutants for OJAP_WD199 and identified homozygous lines with mutations at two independent target sites per gene. Compared to wild-type (WT) plants, ferti...
ORGANISM(S): Oryza sativa Japonica Group 
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